Abstract:

Objective To investigate the antifungal characteristics of fluconazole (FCZ),itraconazole (ITR),voriconazole (VCZ),ketoconazole (KETO),terbinafine (TER),amphotericin B (AmB),miconazole (MIC),5-fluorocytosine (5-FC) to thirty six isolates from tinea pedis.Methods A total of fifty five fungal samples from suspected patients with tinea pedis were examined by microscopy,culture and identification.Minimum inhibitory concentrations (MICs)were determined by Clinical and Laboratory Standards Institute (CLSI)M38-A2 and M27-A3 scheme.Results Thirty six strains of pathogenic fungi were isolated,which were positive both in microscopic examination and culture.They were identified as eight species:Trichophyton rubrum,Trichophyton mentagrophytes,Microsporum flavescens,Trichophyton floccosum,Microsporon lanosum,Candida albicans,Candida glabrata,Candida krusei,and two categories:Dermatophyte and Candida.Dermatophyte and Candida were evaluated with CLSI M38-A2 scheme and M27-A3,respectively.The MICs of eight kinds of antifungal agents to eight kinds of fungi were compared.There were significant differences in MICs of eight kinds of fungi using FCZ,ITR,VCZ,KETO,TER,AMB,MIC and 5-FC (P<0.05).Comparison of the MICs of Dermatophyte and Candida,there were significant differences using FCZ,VCZ,KETO,TER,AMB,MIC,5-FC (P<0.05),except for ITR (P>0.05).The mean rank of TER against Dermatophyte was minimum.Conclusion Eight antifungal agents had significant different antifungal characteristics against eight kinds of fungi.Except ITR,others had significant differences of antifungal characteristics between Dermatophyte and Candida.Comparatively,TER showed the best effect against Dermatophyte.

Key words: tinea pedis, Dermatophyte, Candida, antifungal susceptibility, M38-A2 scheme, M27-A3 scheme