Strain distribution and drug susceptibility results analysis of 1 135 Candida strains

Abstract

Abstract:

Objective To explore the characteristics and drug resisitance of Candida infection in our hospital from Jan.2012 to Oct.2014,to provide aetiological basis for clinical rational use of antifungal agents.Methods Retrospective investigation and analysis method were used in this study.Results A total of 1 135 Candida strains were detected,most of which were C.albicans,followed by C.tropicalis,C.glabrataa,C.parapsilosis and C.krusei.Mainly from the older population,Candida distribution had obvious difference in differentage groups (P<0.05);Men more than women (P<0.05).With sputum specimen was the most,urine second,blood in the third it showed obvious difference in specimen source distribution (P<0.05).Candida infections were mainly from patients with digestive diseases and lung disease.The sensitivity to fluconazol,voriconazole,itraconazole and amphotericin B had statistically difference in different Candida (P<0.05) while the sensitivity to fluorouracil was not statistically significant (P>0.05).Conclusion Candida albicans is the main pathogen of fungal infection followed by tropical Candida.Elderly patients and lung disease patients should guard Candida infections.Fungal isolation,identification and efficacy monitoring are very important to guide clinical rational drug using.Clinical antifungal therapy should be based on the results of strain identification and antifungal susceptibility test.

Key words: Candida, distribution, drug sensitivity test, drug-resistance

CLC Number:

R379.4

DENG Lin, KONG Qing-tao, CHEN Jun, SANG Hong. Strain distribution and drug susceptibility results analysis of 1 135 Candida strains[J]. Chinese Journal of Mycology, 2016, 11(3): 160-165.

References

[1] Clark TA,Hajjeh RA.Recent trends in the epidemiology of invasive mycoses[J].Curr Opin Infect Dis,2002,15(6):569-574.
[2] Denning D W,Munoz P.Advances in invasive fungal infection and antifungal therapy:Introduction[J].Clin Microbiol Infect,2001,7(2):6.
[3] 叶应妩,王毓三.全国临床检验操作规程[M].第2版.南京:东南大学出版社,1997:453.
[4] National committee for clinical laboratory standards.M 27 A.Reference method for broth dilution antifungal susceptbility of yeast,Approved standard[S].Lancaster Avenue,villanova.pennsylvania:NCCLS,1997.1-12.
[5] Mokaddas EM,Al-Sweih NA,Khan ZU.Species distribution and antifungal susceptibility of Candida bloodstream isolates in Kuwait:a 10-year study[J].J Med Microbiol,2007,56(2):255-259.
[6] 廖万清,顾菊林.深部真菌感染治疗的现状与对策[J].中国感染与化疗杂志,2007,7(2):101-103.
[7] Correi A,SamPalo P,Almeida J,et al.Study of molecular epidemiology of candidiasis in Portugal by PCR fingcprrinting of Candida clinical isolates[J].J Clin Mierobiol,2004,42(12):5899-5903.
[8] 阳隽,张天托,朱家馨.白色念珠菌体外生物膜形成与基因分型关系的研究[J].国际检验医学杂志,2012,33(13):1539-1541.
[9] 李政,高玲,王玉春,等.498株念珠菌菌种分布及耐药性分析[J].华夏医学,2013(4):666-669.
[10] 曹凤林,毕铮铮,谷光宇,等.老年糖尿病患者医院感染真菌性肺炎临床分析[J].中华医院感染学杂志,2010,20(7):959-961.
[11] Lyman C A,Navarro E,Garrett KF,et al.Adherence of Candida albicans to bladder mucosa:development and application of a tissue explant assay[J].Mycoses,1999,42(4):255-259.
[12] Bruder-Nascimento A,Camargo CH,Sugizaki MF,et al.Species distribution and susceptibility profile of Candida species in a Brazilian public tertiary hospital[J].BMC Res Notes,2010,3:1.
[13] 赵心懋,徐英春,杨雪松,等.59例假丝酵母菌属菌血症的危险因素与预后分析[J].中华医院感染学杂志,2007,17(9):1169-1170.
[14] Edmond MB,Wallace SE,Mcclish DK,et al.Nosocomial bloodstream infections in United States hospitals:a three-year analysis[J].Clin Infect Dis,1999,29(2):239-244.
[15] Horn DL,Neofytos D,Anaissie EJ,et al.Epidemiology and outcomes of candidemia in 2019 patients:data from the prospective antifungal therapy alliance registry[J].Clin Infect Dis,2009,48(12):1695-1703.
[16] 李从荣,彭少华,李栋,等.深部真菌医院感染的临床调查与耐药现状研究[J].中华医院感染学杂志,2002,12(7):485-487.
[17] 覃开羽,吴敏.589例假丝酵母菌属感染的抗真菌药物分析[J].中华医院感染学杂志,2010,20(16):2522-2523.
[18] 杨继勇,赵慧,张军民.酵母菌引起深部感染的病原学特征[J].中华医院感染学杂志,2003,13(3):284-285.