The amplification and targeting vector construction of a polyketide synthases gene of Fonsecaea monophora

Abstract

Abstract:

Objective To amplify and sequence a polyketide synthases gene of Fonsecaea monophora, and to construct its targeting vector.Methods A polyketide synthases gene of Fonsecaea monophora was amplified and sequenced.Then we designed primers to amplify the 5' and 3' flanking region of PKS gene from Fonsecaea monophora genomic DNA, and the Hygromycin B resistance gene (hyg) from plasmid pBHt1.At last, all fragments were inserted into the vector PDHt/sk.Result The sequence of PKS gene was acquired, and its length was 5 389 bp.The targeting vector of PKS gene was constructed, and the vector was identified by restriction enzyme digestion and nucleotide sequencing.Conclusion This study acquired the targeting vector of PKS gene of Fonsecaea monophora successfully, and laid a good foundation for biological functions research of PKS gene and melanin.

Key words: Fonsecaea monophora, polyketide synthases gene, Agrobacterium tumefaciens, vector construction

CLC Number:

R379.9

XIAO Xing, ZHANG Jun-min, FENG Jiao, CHEN Zhi-wen, CHEN Chun-mei, HE Ya, HE Dan, SUN Jiu-feng, XI Li-yan. The amplification and targeting vector construction of a polyketide synthases gene of Fonsecaea monophora[J]. Chinese Journal of Mycology, 2016, 11(2): 70-74.

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