Effect of baicalin in combination with fluconazole against Candida albicans morphological transition from yeast to hyphae via inhibiting cAMP

Abstract

Abstract:

Objective This study aimed to investigate the antifungal activity of baicalin in combination with fluconazole against Candida albicans morphological transition from yeast to hyphae and to explore the mechanism.Methods The hypha-inducing liquid and solid media were uesd to observe the effect of baicalin alone or in combination with fluconazole on yeast-hapal transformation in C.albicans;The intracellular cAMP level and cAMP rescue was measured by ELISA Kit;Quantitative real time PCR(qRT-PCR) was performed to measure the expression of cAMP-related genes.Results Baicalin in combination with fluconazole could inhibit hyphae formation and intracellular cAMP level obviously;exogenous cAMP could convert hypha-inhibiting effect;And expression of RAS1 and CDC35 were downregulated by 35% and 27% respectively,while the expression of PDE2 was upregulated by 121%.Conclusion Mechanism of baicalin in combination with fluconazole inhibiting C.albicans morphological transition was probabaly via down-regulating the intracellular cAMP.

Key words: C.albicans, baicalin, fluconazole, morphological transition, cAMP

CLC Number:

R379.4

FENG Xin, SHI Gao-xiang, YUN Yun, WU Da-qiang, SHAO Jing, WANG Tian-ming, WANG Chang-zhong. Effect of baicalin in combination with fluconazole against Candida albicans morphological transition from yeast to hyphae via inhibiting cAMP[J]. Chinese Journal of Mycology, 2016, 11(1): 8-12.

References

[1] Martin GS,Maimino DM,Eaton S,et al.The epidemiology of sepsis in the United States from 1979 through 2000[J].N Eng J Med,2003,348(16):1546-1554.
[2] Carlisle PL,Banerjee M,Lazzell A,et al.Expression levels of a filament-specific transcriptional regulator are sufficient to determine Candida albicans morphology and virulence[J].Proc Natl Acad Sci U S A,2009,106(2):599-604.
[3] Chou TC,Chang LP,Li CY,et al.Theamtiinflammatory and analgesic effects of baicalin in carrageenan-evoked thermal hyperalgesia[J].Ansth Analg,2003,97(6):1724-1729.
[4] 熊英,傅颖媛,况南珍,等.黄芩苷抗白念珠菌作用机制研究[J],中国药理学通报,2004,20(12):1404-1407.
[5] Wang TM,Shi GX,Shao J,et al.In vitro antifungal activity of baicalin against Candida albicans biofilms via apoptotic induction[J].Micro Patho,2015,87:21-29.
[6] 颜贵明,施高翔,邵菁,等.黄芩苷联合氟康唑对白念珠菌生物膜的抑制作用研究[J].中国真菌学杂志,2015,10(3):139-145.
[7] 陈新辉,赵明玄,安金刚,等.单克隆天然抗角蛋白抗体IgM3B4体外抑制白念珠菌芽管形成及黏附作用[J].第四军医大学学报,2008,29(1):47-50.
[8] Maidan MM,De Rop L,Semeels J,et al.The G protein-coupled receptor Gprl and the Ga protein Gpa2 act through the cAMP-protein kinase A pathway to induce morphogenesis in Candida albicans[J].Mol Biol Cell,2005,16(4):1971-1986.
[9] Jung WH,Stateva LI.The cAMP phosphodiesterase encoded by CaPDE2 is required for hyphal development in Candida albicans[J].Microbiology,2003,149(Pt 10):2961-2976.
[10] Xie Z,Thompson A,Kashleva H,et al.A quantitative real-time RT-PCR assay for mature C.albicans biofilms[J].BMC Microbiol,2011,11:93.
[11] Hogan DA,Sundstrom P.The Ras/cAMP/PKA signaling pathway and virulence in Candida albicans[J].Future Microbiol,2009,4(10):1263-1270.

[1]	HUANG Jin-bao, LAN Chang-qing, WEN Heng, LIN Qing-hua. Cryptococcal infection of the ribs mimicking malignant tumor: two cases report and literature review [J]. Chinese Journal of Mycology, 2020, 15(5): 278-282.
[2]	CAI Qing, YANG Lian-juan, GAO Zhi-qin, REN Hong-jin, YANG Hong, TAN Jing-wen. In vitro study of the effect of romidepsin on fluconazole againgst Candida albicans [J]. Chinese Journal of Mycology, 2019, 14(4): 200-203,216.
[3]	LIU Fei-fei, WANG Yan, CHEN Rong, SHEN Jian-zhen. Clinical efficacy and safety of intravenous injection voriconazole and fluconazole in preventing fungal infection in China: A meta analysis [J]. Chinese Journal of Mycology, 2019, 14(3): 159-163.
[4]	LEI Yan, FANG Wei, ZHANG Chao, LIAO Wan-qing. In vitro chessboard detection for anti-Cryptococcus effect of rattlerin and fluconazole [J]. Chinese Journal of Mycology, 2019, 14(2): 90-94.
[5]	LIU Fei-fei, WANG Qin-qin, WANG Yu, SHEN Jian-zhen. Clinical efficacy of oral fluconazole combined with clotrimazole suppositories in the treatment of Candida vaginitis: a meta analysis [J]. Chinese Journal of Mycology, 2018, 13(5): 257-261.
[6]	YANG Min, XIE Shuang, DONG Yu, DU Yong-hong, LI Dai-rong. Evaluation of fungicidal effect of low frequency ultrasound and amphotericin B-loaded nanoparticles on C. albicans biofilm [J]. Chinese Journal of Mycology, 2018, 13(3): 134-138.
[7]	WANG Yu-lian, YUAN-Gong, CHEN Ya-nan, CAO Yong-bing, WU Jian-hua. The effect of rifapentine and fluconazole on the cell cycle of FLC-resistance Candida albicans [J]. Chinese Journal of Mycology, 2018, 13(3): 144-147.
[8]	YOU Zi-meng, RAN Xin, DAI Ya-ling, RAN Yu-ping. Clinical and experimental research of using topical clioquinol cream to treat infantile candidal intertrigo [J]. Chinese Journal of Mycology, 2018, 13(1): 26-29.
[9]	XIE Jun, JIN Qin, WU Dan. Recurrent genital 45 cases of vaginal Candida disease clinical treatment experience [J]. Chinese Journal of Mycology, 2017, 12(3): 152-155.
[10]	DONG Huai-huai, WANG Yuan-hua, LIAO Ze-bin, JIANG Yuan-ying, CAO Ying-ying. Synergistic effect of mangiferin in combination with fluconazole against fluconazole-resistant Candida albicans [J]. Chinese Journal of Mycology, 2017, 12(2): 78-82,85.
[11]	WANG Li-hong, ZHANG Jin-yu, YAN Lan, JIANG Yuan-ying, CAO Yong-bing, HONG Hao. The transcription factors of phenotypic transition signal pathways in Candida albicans [J]. Chinese Journal of Mycology, 2017, 12(2): 114-119.
[12]	SHI Gao-xiang, WANG Yun-xia, FENG Xin, SHAO Jing, WANG Tian-ming, WANG Chang-zhong. Butyl alcohol extract of BaiTouWeng decoction induced apoptosis in Candida albicans biofilms [J]. Chinese Journal of Mycology, 2017, 12(1): 13-18.
[13]	WANG Yu-lian, ZHANG Lu-lu, QIN Yu-lin, CAO Yong-bing, WU Jian-hua. Synergistic effect of rifapentine with fluconazole against FLC-resistance Candida albicans [J]. Chinese Journal of Mycology, 2016, 11(3): 156-159.
[14]	YAN Gui-ming, Shi Gao-xiang, SHAO Jing, WANG Tian-ming, XIA Dan, WANG Chang-zhong. Inhibitory effects of baicalin in combination with fluconazole against Candida albicans biofilms [J]. Chinese Journal of Mycology, 2015, 10(3): 139-145.
[15]	Xu Hui, CHENG Hong-xia, ZOU Xian-biao. Inhibitory effect of different durations of incubation of a photosensitizer on photodynamics of C.albicans [J]. Chinese Journal of Mycology, 2015, 10(3): 146-150.