MLPA application in clinical diagnosis of pathogen

Abstract

Abstract:

As for patients diagnosed with Infectious diseases, favorable prognosis is determined by correct diagnosis and proper treatment.So it is important to diagnose clinical pathogens rapidly.Compared with other clinical test methods, MLPA is a simple, valid, and fast one which can detect several gene sequences in quantity at the same time.The steps include:the degeneration of DNA sample, hybridization, ligation, and PCR amplification.Although being at its first stage, there are already certain applications of MLPA in diagnosing and researching of clinic microorganism, and the peculiarity of easy operation, fast reaction, high pass rate and high sensitivity have reveal bright prospect of MLPA.

Key words: multiplex ligation-dependent probe amplification, pathogen, diagnosis

CLC Number:

R446.5

FANG Wen-jie, LIU Jia, PAN Wei-hua, Liao Wanqing. MLPA application in clinical diagnosis of pathogen[J]. Chinese Journal of Mycology, 2015, 10(5): 307-311.

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