Abstract:

Objectives We reported a girl with kerion due to Trichophyton rubrum.By molecular diagnosis of affected hairs, the pathogenic isolate was identifiedso that direct treatment could be executed.Methods As well as direct microscopic examination and fungal culture of affected hairs, fungal DNA of them was extracted, and amplification of ITS and sequencing.Results The molecular identification of affected hairs revealed T.rubrum, which was in accordance with fungal culture from the affected hairs.The patient was diagnosed as kerion due to T.rubrum and treated with oral terbinafine 125 mg per day.The lesions subsided after 1 month.Conclusion PCR-based molecular diagnosis of DNA from hair could help to identify pathogenic fungus promptly and indicate proper antifungal treatment.

Key words: T.rubrum, kerion, culture-independent diagnosis