The application of nucleic acid sequence-based amplification,real-time PCR and GM test in invasive aspergillosis diagnosis

Abstract

Abstract:

Objective To study the diagnostic performance of nucleic acid sequence-based amplification(NASBA) assay, real-time PCR and GM test in detecting invasive aspergillosis for clinical diagnosis.Methods Blood samples from 80 patients at a high risk for IA were collected during from November 2013 to June 2014.These patients were categorized as 8 proven IA, 26 probable IA, and 46 non-IA according to the 2008 revised definitions of EORTC/MSG.Blood samples were tested by NASBA, real-time PCR and GM test and their diagnostic parameters were calculated, respectively.Result The sensitivity of NASBA, real-time PCR and GM test was 76.47%, 67.65% and 52.94%, while their specificity was 80.43%, 89.13%, 80.43%, respectively.The efficiency of various combinations of tests was also evaluated.Perfect specificity(100%) and positive predictive value(100%) were achieved by combining NASBA and real-time PCR as a serial testing.A combination of NASBA and real-time PCR as a parallel testing was the most sensitive(94.12%).Conclusion The sensitivity and specificity of NASBA and real-time PCR were superior to GM test.Combination of these assays could be particularly useful in specific clinical situations.

Key words: invasive aspergillosis, nucleic acid sequence-based amplification, real-time PCR, GM test

CLC Number:

R379.6

WANG Li-peng, BAO Cui-xia, YU Li-mei, ZHANG Xiao-lu, YU Wei-juan, ZHANG Xia, LI Wei, HUANG Bao-hua, LI Jie, Sun Chengming. The application of nucleic acid sequence-based amplification,real-time PCR and GM test in invasive aspergillosis diagnosis[J]. Chinese Journal of Mycology, 2015, 10(5): 283-287.

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