Establishment of a rat model of acute invasive fungal rhinosinusitis

Abstract

Abstract:

Objective To develop a rat model of acute invasive fungal rhinosinusitis(AIFR) which is stable and easy to operate in order to promote the basic and clinical researches of AIFR.Methods Sprague-Dawley(SD) rats were divided randomly into four groups.Immunosuppression, right nasal packing Merocel sponge and nasal inoculation with Aspergillus fumigatus spores were all involved in group A.Only right nasal packing Merocel sponge and nasal inoculation with A.fumigatus spores were involved in group B.Only immunosuppression and nasal inoculation with A.fumigatus spores were involved in group C.No treatment was involved in group D.A.fumigatus was dropped into rat nasal for three consecutive days in group A, B and C.All rats were killed four days after the first fungi intranasal instillation.Hematology, fungal culture and histopathology investigations were performed.Results The neutrophil quantities reduced after immunosupression, and less than 0.1×10⁹/L on the first administration day of A.fumigatus spores.An AIFR rat model was established successfully only in group A with an incidence rate of 90%(9/10).A.fumigatus invasion was also observed in 10%(1/10) of the lungs in group A and 20%(2/10) in group C.Positive rates of fungal culture of nasal tissue was about 71%(5/7) in group A, while the remaining groups was zero.Conclusion Immunosupression, nasal obstruction, and fungal inoculation were the three essential conditions for successfully developping a stable AIFR rat model.This model is stable, easy to operate, and closely mimics the pathophysiology of anthropic AIFR.It can be used in a further study in immunity, pathology, drug of AIFR.

Key words: invasive rhinosinusitis, Aspergillus fumigatus, rat model

CLC Number:

R379.6

YAN Yu-yan, ZHAO Zuo-tao, Liu Honggang. Establishment of a rat model of acute invasive fungal rhinosinusitis[J]. Chinese Journal of Mycology, 2015, 10(5): 261-265,278.

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