Abstract:

Objective To research the sensitivity of major Candida,isolated from severe vulvovaginal candidiasis to terconazole.Methods Isolates were collected from the patients with vulvovaginal candidiasis,who were from the Gynecology clinic of Shanghai Renji Hospital,Shanghai Sixth People's Hospital and Shanghai First Maternity and Infant Hospital.CHROM agar candida medium and API 20C AUX were used to identify Candida species.The sensitivity of 213 Candida isolates to terconazole,clotrimazole and miconazole were tested by microdilution broth method according to the CLSI M27-A3 microdilution method.Results 192 isolates were identified to C.albicans (90.14%),21 isolates were identified to C.glabrata (9.86%).The sensitivity of C.albicans isolates to terconazole were as follows:MIC range:0.024-12.5 μg/mL,MIC₅₀:0.39 μg/mL,MIC₉₀:1.56 μg/mL,GM:0.248 μg/mL;The sensitivity of C.glabrata isolates to terconazole were as follows:MIC range:0.024-1.56 μg/mL,MIC₅₀:0.049 μg/mL,MIC₉₀:1.56 μg/mL,GM:0.107 μg/mL.Conclusions Currently vaginal candidiasis infection is still based on C.albicans.The research reprompted terconazole with clotrimazole,miconazole three kinds of antifungal drugs on the 213 Candida have good sensitivity.Terconazole has strong inhibition to C.albicans and C.glabrata and did not find resistance phenomenon.

Key words: CLSIM27-A3, antifungal sensitivity, vulvovaginal candidiasis, terconazole, clotrimazole, miconazole