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Chinese Journal of Mycology 2015, Vol. 10  Issue (3): 146-150.

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Inhibitory effect of different durations of incubation of a photosensitizer on photodynamics of C.albicans

Xu Hui1, CHENG Hong-xia2, ZOU Xian-biao2   

  1. 1. Chinese PLA Medical School, Beijing 100853, China;
    2. Department of Dernatlogy, First Affiliated Hospital of Chinese PLA General Hospital, Beijng 100048, China
  • Received:2014-12-18 Online:2015-06-28 Published:2015-06-28

Abstract:

Objective To facilitate the selection of the best lighting time by determining the level at which C.albicans and 5-ALA PDT generate protoporphyrin IX (PpIX) at different time points and by measuring the bacteriostatic rate of 5-amino ketones pentanoic acid photodynamic therapy (5-ALA PDT).Method C.albicans suspension was prepared and incubated with ALA in the dark.The consequent mixture was divided into the control group and experimental group.The generation of PpIX was observed under a laser confocal microscope.The inhibitory rate of 5-ALA PDT on C.albicans was determined by MTT method.Results After dark incubation,C.albicans and ALA produced fluorescent PpIX that reached the peak amount between 30 min and 90 min,but began to decrease significantly at 120 min.There was no fluorescent material in control group.Conclusion The inhibitory effect of ALA PDT on C.albicans was closely related to the concentration of the photosensitizer,which provides data for treatment of C.albicans disease.

Key words: 5-amino ketones pentanoic acid, photodynamic, C.albicans, drug concentration

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