Construction and application of blood-brain-barrier model in vitro in cryptococcal infection

Abstract

Abstract:

Objective To establish an in vitro Blood-Brain-Barrier (BBB) model,which was then applied to evaluate the capacities of different cryptococcal strains in crossing Blood-Brain-Barrier.Methods Mouse brain endothelium cell bEND.3 was used to construct the Blood-Brain-Barrier model in vitro.In order to test its feasibility,we next examined the efficacy of several cryptococcal strains with different serotype or gene mutation in central nervous system (CNS) invasion.Results The transendothelial electrical resistance (TEER) measurement suggested that BBB model was constructed successfully.Compared with C.neoformans serotype A strain H99,JEC21 (C.neoformans serotype D) displayed a reduced efficacy in BBB invasion while the negative control strain (non pathogenic Saccharomyces cerevisiae strain Y187) with the lowest efficacy.Furthermore,the ure1 mutant (urease deletion) and the cap59 mutant (capsule deletion) showed a 50% and 80% decrease of their efficacy in crossing BBB compared to their parent strain H99,respectively (P<0.001).Conclusion The in vitro model of cryptococcal CNS infection was established successfully.Serotype,urease,and capsule was essential for C.neoformans to cross the Blood-Brain-Barrier.

Key words: Cryptococcus neoformans, Blood-Brain-Barrier, in vitro model, pathogenesis

CLC Number:

MENG Yun-fang, FA Zhen-zong, FANG Wei, ZHOU Zhao-jing, YI Jiu, GU Ju-lin, LIAO Wan-qing. Construction and application of blood-brain-barrier model in vitro in cryptococcal infection[J]. Chinese Journal of Mycology, 2015, 10(2): 92-95.

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