Analysis of relationship between transcription factor RPN4 and drug susceptibility of Candida albicans

Abstract

Abstract:

Objective To study the influence on the drug susceptibility of Candida albicans after transcription factor RPN4 was knocked out and the mechanism how this transcription factor regulates the drug susceptibility in Candida albicans.Methods The broth microdilution method was used to determine the minimal inhibitory concentrations (MICs) of 30 strains transcription factor knocked out Candida albicans .The drug susceptibility was reassured by spot assay and growth curve.The total RNA of parental strain SN250 and susceptible strains were extracted and the expression of multidrug resistance genes CDR1 and MDR1 was determined by real-time PCR,and the pump ability was examined by the ability of pump out rhodamine 6G.Results Compaired to the parental strain SN250,transcription factor RPN4 knocked out strain was more susceptible to fluconazole.The expression of CDR1 and MDR1 was lower than SN250 and the pump ability was also weaker than SN250.Conclusion The knockout of transcription factor PRN4 results susceptible to fluconazole in Candida albicans,and this transcription factor may be involved in the regulation of drug susceptibility in Candida albicans.

Key words: Candida albicans, transcription factors, drug susceptibility, multidrug resistance genes

CLC Number:

R379.4

XU Hong-tao, TANG Ruo-yu, QU Ying-ge, WANG Xiao-juan, JIANG Yuan-ying, CAO Yong-bing, YAN Tian-hua. Analysis of relationship between transcription factor RPN4 and drug susceptibility of Candida albicans[J]. Chinese Journal of Mycology, 2015, 10(2): 75-79.

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