The morphological diversity and virulence factorssecretory activity of Candida parapsilosis

Abstract

Abstract:

Objective Compared the colonies and cells morphology and the secreted aspartic proteinase (SAP) enzyme activity of different clinical isolates of Candida parapsilosis,and elucidated the morphological diversity and the differences of virulence of the pathogenic fungi.Method Seven clinical isolates of Candida parapsilosis were cultured respectively on different solid media,and the morphology characteristics of colonies and cells were observed and recorded.Strains were also cultured on solid medium of bovine serum albumin (BSA) at 30 ℃ for determining the secretory activity of the virulence factors (SAP) by observing the proteolytic halos.Result Diverse colonies and phenotypes of the above strains were checked under several sorts of conditions—smooth,crepe and crater in various shades of stained color,and the ability of filamentation were also different from each other among the tested strains.In addition,the sizes of proteolytic halos were various from 2.55 cm to 1.55 cm.Conclusion There are diverse phenotypes which can be shown for the clinical isolates of Candida parapsilosis,and the SAP enzyme activities of them are distinct,which may imply the significant virulent differences among the strains.

Key words: Candida parapsilosis, Morphological diversity, SAP activity

CLC Number:

R379.4

LI Xiao-ling, JIN Fang, CHEN Yu-ru, MU Li-li, LIU Tao-hua, LV Qian, KANG Ying-qian. The morphological diversity and virulence factorssecretory activity of Candida parapsilosis[J]. Chinese Journal of Mycology, 2015, 10(2): 65-69.

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