New advance in the study of the molecular biological identification and typing of Prototheca

Abstract

Abstract:

The molecular biology method based on gene sequencing which is objective,highly reproducible and so on,is considered the "gold standard" of Prototheca's molecular identification.To summarize the new molecular biology methods and techniques applied to Prototheca's identification and typing,such as real-time quantitative PCR (RT-PCR) technology,single strand conformation polymorphism (SSCP) analysis,PCR-restriction fragment length polymorphism (RFLP)analysis,high resolution melting (HRM) analysis,mass spectrometry(MS) analysis.

Key words: Prototheca, Molecular biology, PCR, MS

CLC Number:

R519.5

LI Wen-jing, LIU Jin-yan, SHI Ce, WANG Ying, SUN Jing-yong, XIANG Ming-jie. New advance in the study of the molecular biological identification and typing of Prototheca[J]. Chinese Journal of Mycology, 2015, 10(2): 126-128.

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