Anti-adhesion effect of Butyl alcohol extract of BaiTouWeng decoction on Candida albicans isolated from VVC

Abstract

Abstract: Objective To investigate anti-adhesion effect of Butyl alcohol extract of BaiTouWeng decoction (BAEB)on Candida albicans.Methods Agar plate assay was used to determine CFU of Candida albicans for 2h and 4h.XTT reduction assay was applied to evaluate the adherent Candida albicans merabolic activity.Test tube assay was utilized to evaluate flocculation.The water-hydrocarbon two-phase assay was employed to measure the cell surface hydrophobicity of Candida albicans.Scanning electron microscope(SEM)and fluorescein diacetate(FDA)staining was used to observe the morphological changes of adherent Candida albicans.qRT-PCR was adopted to inspect the expression of adhesion-related genes such as INT1,ALS3,MNT2,ALA1,ALS1,ALS5,HWP1 and CSH1.Results 512,1 024 μg/mL BAEB could effectively inhibit adherence of Candida albicans.Flocculation was delayed by 512,1 024 μg/mL BAEB.The cell surface hydrophobicity in the BAEB treated groups was lower than that of the control group.The expression of ALS1,CSH1,ALA1,ALS3,INT1,HWP1 were downregulated dramatically after BAEB treatment,but ALS5 and MNT2 were unchanged.Conclusion BAEB could effectively inhibit adherence of Candida albicans isolated from VVC by regulating adhesion-associated genes.

Key words: butyl alcohol extract of BaiTouWeng decoction (BAEB), vulvovaginal candidiasis(VVC), Candida albicans, adhesion

CLC Number:

R379.4

ZHANG Meng-xiang, XIA Dan, SHI Gao-xiang, LU Ke-qiao, SHAO Jing, WU Da-qiang, WANG Tian-ming, WANG Chang-zhong. Anti-adhesion effect of Butyl alcohol extract of BaiTouWeng decoction on Candida albicans isolated from VVC[J]. Chinese Journal of Mycology, 2015, 10(1): 25-30.

References

[1] 乐杰,谢幸,林仲秋,等.妇产科学[M].第7版.人民卫生出版社,2008:239-240.
[2] 张瑾,高月平.中药治疗阴道炎研究进展[J].实用中医药杂志,2009,25(2):135-136.
[3] 于晓虹,杨国玲,刘晓明,等.氟康唑、特比萘芬和伊曲康唑对白色念珠菌的体外敏感性试验[J].中国皮肤性病学杂志,2008,22(11):668-669.
[4] 张文平,王小丽,黄真,等.肉桂醛体外诱导白色念珠菌耐药的实验[J].中国临床康复,2006,10(35):148-149.
[5] 王刚生,邓洁华.中药提取组合物(胶囊)对念珠菌及曲霉的体外抗菌实验[J].时珍国医国药,2010,21(4):884-886.
[6] 张晓芬,张超云.白头翁汤加味综合治疗复发性念珠菌性阴道病[J].中国实验方剂学杂志,2012,18(9):279-281.
[7] 赵柳娅,蒋京辰,姜远英,等.黄芩素与氟康唑协同抗白念珠菌生物被膜作用研究[J].中国真菌学杂志,2014,9(2):70-74.
[8] Gelis S,de Groot PW,Castillo L,et al.Pga13 in Candida albicans is localized in the cell wall and influences cell surface properties,morphogenesis and virulence[J].Fungal Genet Biol,2012,49(4):322-331.
[9] Sundstrom P.Adhesion in Candida spp[J].Cell Microbiol Cell Microbiol,2002,4(8):461-469.
[10] Gaur NK,Klotz SA.Expression,cloning,and characterization of a Candida albicans gene,ALA1,that confers adherence properties upon Saccharomyces cerevisiae for extracellular matrix proteins[J].Infect Immun,1997,65(12):5289-5294.
[11] Asleson CM,Bensen ES,Gale CA,et al.Candida albicans INT1-induced filamentation in Saccharomyces cerevisiae depends on Sla2p[J].Mol Cell Biol,2001,21(4):1272-1284.
[12] Gaur NK,Smith RL,Klotz SA.Candida albicans and Saccharomyces cerevisiae expressing ALA1/ALS5 adhere to accessible threonine,serine,or alanine patches[J].Cell Commun Adhes,2002,9(1):45-57.
[13] Spreghini E,Gismondi A,Piccoli M,et al.Evidence for alphavbeta3 and alphavbeta5 integrin-like vitronectin(VN)receptors in Candida albicans and their involvement in yeast cell adhesion to VN[J].J Infect Dis,1999,180(1):156-166.
[14] Fu Y,Ibrahim AS,Sheppard DC,et al.Candida albicans Als1p:an adhesin that is a downstream effector of the EFG1 filamentation pathway[J].Mol Microbiol,2002,44(1):61-72.
[15] De Souza RD,Mores AU,Cavalca L,et al.Cell surface hydrophobicity of Candida albicans isolated from elder patients undergoing denture-related candidosis[J].Gerodontology,2009,26(2):157-161.
[16] Kevin J,Verstrepen,Frans M Kils.Flocculation,adhesion and biofilm formation in yeasts[J].Mol Microbiology,2006,60(1):5-15.