The research progress of Candida albicans wall-breaking method

Abstract

Abstract: Cell wall as a special and necessary cell structure of fungus.it's hard to make it fully broken in a simple way. Therefore, to achieve the ideal wall-breaking effect is one of the key steps in the study of Candida albicans. In extraction such as RNA, DNA and protein components of Candida albicans, in order to obtain sufficient and stable experimental samples, the researchers shoulduse different methods breaking the cell wall according to different research purposes. This paper summarizes a variety of Candida albicans wall-breaking methods, so as to provide applicable and efficient solution of wall-breaking for related research in Candida albicans.

Key words: Candida albicans, cell wall, breaking

CLC Number:

R379.4

WANG Xiao-fei, ZHANG Lu-lu, CAO Yong-bing, JIANG Yuan-ying. The research progress of Candida albicans wall-breaking method[J]. Chinese Journal of Mycology, 2014, 9(5): 310-315.

References

[1] Yan L,Li M,Gao P,et al.The alternative oxidase of Candida albicans causes reduced fluconazole susceptibility[J].J Antimicrob Chemother,2009,64(4):764-773.
[2] 张石群,曹永兵,姜远英.白念珠菌细胞壁相关基因和蛋白的研究进展[J].中国真菌学杂志,2011,6(4):243-246.
[3] Chaffin WL,López-Ribot JL,Casanov M,et al.Cell wall and secreted proteins of Candida albicans:identification,function,and expression[J].Microbiol Mol Biol Revi,1998,62(1):130-180.
[4] Cassone A.Cell wall of Candida albicans: its functions and its impact on the host[J].Curr Top Med Mycol,1989,3(1):248-314.
[5] Tokunaga M,Kusamichi M,Koike H.Ultrastructure of outermost layer of cell wall in Candida albicans observed by rapid-freezing technique[J].J Electron Microsc,1986,35(3):237-246.
[6] Klis FM,De Groot P,Hellingwerf K.Molecular organization of the cell wall of Candida albicans[J].Med Mycol,2001,39(1):1-8.
[7] Pitarch A,Sánchez M,Nombela C,et al.Analysis of the Candida albicans proteome I. Strategies and applications[J].J Chromatography B,2003,787(1):101-128.
[8] Lenardon MD,Whitton RK,Munro CA,et al.Individual chitin synthase enzymes synthesize microfibrils of differing structure at specific locations in the Candida albicans cell wall[J].Mol Microbiol,2007,66(5):1164-1173.
[9] Lim CS,Tung CH,Rosli R,et al.An alternative Candida spp. cell wall disruption method using a basic sorbitol lysis buffer and glass beads[J].J Microbiol Methods,2008,75(3):576-578.
[10] Cardinali G,Bolano A,Martini A.A DNA extraction and purification method for several yeast genera[J].Annals of Microbiology,2001,51(1):121-130.
[11] Karakousis A,Tan L, Ellis D,et al.An assessment of the efficiency of fungal DNA extraction methods for maximizing the detection of medically important fungi using PCR[J].J Microbiol Methods,2006,65(1):38-48.
[12] 段德鉴,蒋献,冉玉平,等.白念珠菌对角质形成细胞β防御素-2mRNA表达的影响[J].中华皮肤科杂志,2005,38(4):202-204.
[13] Trinel PA,Borg-Von-Zepelin M,Lepage G,et al.Isolation and preliminary characterisation of the 14 to 18 kilodalton Candida albicans antigen as a phospholipomannan containing β-1,2-linked oligomannosides[J].Infect Immun,1993,61(1):4398-4405.
[14] 王仕钦,曹王丽,李芳秋.白念珠菌烯醇化酶重组蛋白质的制备及鉴定[J].临床检验杂志,2010,28(5):353-355.
[15] 王萍,厉荣玉,董群.白假丝酵母菌DNA提取方法的研究[J].热带医学杂志,2008,8(12):1222-1224.
[16] Silverman RJ, Nobbs AH, Vickerman MM,et al.Interaction of Candida albicans cell wall Als3 protein with Streptococcus gordonii SspB adhesin promotes development of mixed-species communities[J].Infect Immun,2010,78(11):4644-4652.
[17] Yordanov M, Tchorbanov A, Ivanovska N.Candida albicans cell wall fraction exacerbates collagen-induced arthritis in mice[J].Scand J Immunol,2005,61(4):301-308.
[18] Li XS, Sun JN, Okamoto-Shibayama K,et al.Candida albicans Cell Wall Ssa Proteins Bind and Facilitate import of Salivary Histatin 5 Required for Toxicity[J].J Biol Chem,2006,281(32):22453-22463.
[19] Chaffin WL, López-Ribot JL, Casanova M,et al.Cell wall and secreted proteins of Candida albicans:identification,function, and expression[J].Microbiol Mol Biol Rev,1998,62(1):130-180.
[20] Sun S,Lou H,Gao Y,et al.Liquid chromatography-tandem mass spectrometric method for the analysis of fluconazole and evaluation of the impact of phenolic compounds on the concentration of fluconazole in Candida albicans[J].J Pharm Biomed Anal,2004,34(5):1117-1124.
[21] 冷萍,郭秀丽,邢杰,等.羽苔素E对白念珠菌细胞内氟康唑浓度的影响研究[J].药物分析杂志,2009,29(4):564-568.
[22] 刘洪涛,高平挥,曹永兵,等.气-质联用研究氟康唑对白念珠菌甾醇生物合成的抑制作用[J].中国药理学与毒理学杂志,2002,16(5):368-371.
[23] Mora-Montes HM, Netea MG, Ferwerda G,et al.Recognition and blocking of innate immunity cells by Candida albicans chitin[J].Infect Immun,2011,79(5):1961-1970.
[24] Castro M, Ralston NV, Morgenthaler TI,et al.Candida albicans stimulates arachidonic acid liberation from alveolar macrophages through α-mannan and β-glucan cell wall components[J].Infect Immun,1994,62(8):3138- 3145.
[25] 周村建,郝飞,王鲁,等.白念珠菌总RNA提取的实验研究[J].中华皮肤科杂志,2004,37(8):494-495.
[26] 陈官芝,王云,徐秀莲,等.致病性真菌DNA提取方法的实验研究[J].中华皮肤科杂志,2002,35(5):408-409.
[27] Sosinska GJ, de Koning LJ, de Groot PW,et al.Mass spectrometric quantification of the adaptations in the wall proteome of Candida albicans in response to ambient pH[J].Microbiology,2011,157(1):136-146.
[28] Hazen KC,BW Hazen. Hydrophobic surface protein masking by the opportunistic fungal pathogen Candida albicans[J].Infect Immun,1992,60(4):1499-1508.
[29] de Groot PW, de Boer AD, Cunningham J,et al.Proteomic analysis of Candida albicans cell walls reveals covalently bound carbohydrate-active enzymes and adhesins[J].Eukaryotic Cell,2004,3(4):955-965.
[30] Kapteyn JC, ter Riet B, Vink E,et al.Low external pH induces HOG1-dependent changes in the organization of the Saccharomyces cerevisiae cell wall[J].Mol Microbiol,2001,39(2):469-479.
[31] Singleton DR,Masuoka J,Hazen KC.Cloning and analysis of a Candida albicans gene that affects cell surface hydrophobicity[J].Bacteriol,2001,183(12):3582-3588.