Detection of helper T cells and regulatory T cells of 21 cases with invasive pulmonary aspergillosis patients

Abstract

Abstract: Objectives To analysis CD4⁺T cells in peripheral blood mononuclear cell and clinical data of invasive pulmonary aspergillosis(IPA) patients and explore function of cellular immunity mediated by helper T cells and regulatory T cells in IPA.Methods A total of 21 IPA patients were evaluated. 19 healthy volunteers as the normal control group. We detected Th1,Th2,Th17 and Treg expression in CD4⁺T cells, related cytokine secretion and key transcription factors of IPA patients and controls.Results Patients with IPA exhibited decreased Th1 and Treg expression, related cytokines (IFN-γ, IL-17A, TGF-β) and T-bet, RORγt and Foxp3 mRNA levels when compared with the normal control group. while there was no significant difference between patients with IPA and the normal control group about peripheral Th2 frequency, Th2 related cytokines IL-4, and GATA-3 mRNA levels.Conclusions Th1,Th17and Treg-mediate cellular immunity were inhibited in IPA patients.

Key words: invasive pulmonary aspergillosis, helper T cells, interleukine-17, interferon-γ

CLC Number:

R519.8

WANG Run-chao, WAN Zhe, LI Ruo-yu. Detection of helper T cells and regulatory T cells of 21 cases with invasive pulmonary aspergillosis patients[J]. Chinese Journal of Mycology, 2014, 9(5): 287-292.

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