The identification of 94 clinical isolates from vulvovaginal candidiasis in Fujian area

Abstract

Abstract: Objective To reclassify the 94 Candida strains from clinical vulvovaginal Candidiasis patients in Fujian area, and to evaluate the accuracy of CHROM agar identification by comparing with the API test and molecular biological analysis.Methods 1Identify the 94 strains by CHROM agar, and observe the microscopic morphology. 2Identify these strains by ITS sequence analysis of molecular biological method. 3Comparing the test reports from the clinical laboratory with the identification results of CHROM agar and ITS sequence analysis, the isolates in doubt are selected to have the API test and LSU sequence analysis.Results 1There were 78 strains identified as C.albicans, 10 strains as C.glabrata, 3 strains as C.parasilosis, 1 strain as Saccharomyces cerevisiae, and 1 strains as both C.glabrata and C.parasilosis. 2The CHROM agar could nearly correctly identify Candida, however, lacks specificity when it comes to rare isolates (ex.Saccharomyces cerevisiae). 3According to the comparing results, we could conclude that the identification of clinical laboratory just by the methods of simple morphology and CHROM agar test still has the error rate (10/94). 4The identification of molecular biological method had high accuracy rate. Besides, we could identify the C.parapsilosis complex was consist of the species of C.parapsilosis sensu strico,Candida metapsilosis and Candida orthopsilosis by this way.Conclusions The most part of clinical isolates of vulvovaginal candidiasis in Fujian area were still C.albicans, but the non C.albicans also occupied a certain proportion (16/94). Our result showed that the molecular biological methods were more accurate than the CHROM agar test in identification.

Key words: candidiasis, identification, molecular biology, CHROM agar

CLC Number:

R519.3

CHEN Bo-rui, JIANG Ai-ping, XU Ming, LU Wan-ding, CAI Lei. The identification of 94 clinical isolates from vulvovaginal candidiasis in Fujian area[J]. Chinese Journal of Mycology, 2014, 9(4): 222-227.

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