Abstract:

Objective To find the influence of culture medium with different kinds of nitrogen and carbon source on the growth morphology of Trichophyton rubrum.Method Point inoculated the Trichophyton rubrum on potato dextrose medium,trypsin agar medium, keratin glucose medium and glucose agar medium. Observed the morphology and tested the area of the colony after 2 weeks of incubation. Compared the growth morphology of Trichophyton rubrum when inoculated with and without glucose. Compared the influence of different keratin concentration to the growth morphology of Trichophyton rubrum.Result Different protein as nithogen source in different medium could all promote the growth of Trichophyton rubrum.Yet Trichophyton rubrum growed better in keratin culture and produced more red pigment. Glucose added to the keratin culture as carbon source could effectively promote the growth of Trichophyton rubrum and the production of red pigment.Conclusion The concentration of keratin in the culture medium can affect the growth of Trichophyton rubrum,and the glucose is the key factor to the production of red-pigment for Trichophyton rubrum. The overall understanding of metabolic mechanism of Trichophyton rubrum might provide a good basis for further study and even a good strategy of the antifungal research.

Key words: Trichophyton rubrum, keratin, glucose, red pigment