Eeffect of Fonsecaea monophora on the expressions of TLR2,TLR4,Dectin-1 and the production of TNF-α in macrophages

Abstract

Abstract:

Objective To explore the effect of F.monophora on the expressions of toll-like receptor 2 (TLR2),toll-like receptor 4 (TLR4),C-type lectin-1 (Dectin-1) and the production of proinflammatory cytokine tumor necrosis factor-α (TNF-α) in macrophages.Method Murine macrophages (RAW264.7) were co-cultured with the meristematic mutant (CBS122845)and the albino mutant (CBS125149)of F.monophora. RAW264.7 macrophages were used as negative control. We detected the mRNA expressions of TLR2, TLR4, Dectin-1 and MyD88, NF-κB by real-time PCR (RT-PCR) and detected the protein expressions of TLR2, TLR4, Dectin-1 by Flow CytoMetry (FCM). The protein expression of TNF-α was measured by ELISA.Results Compared with the negative control group, the mRNA and protein expressions of TLR2, TLR4, Dectin-1 and MyD88, NF-κB increased in CBS122845 and CBS125149 group. Compared to CBS125149 group, the mRNA and protein expression of TLR2 in CBS122845 group had no significant change, while the mRNA and protein expressions of TLR4, Dectin-1 and MyD88, NF-κB increased more significantly.Compared with the control group, CBS122845 group significantly decreased the protein secretion of TNF-α cytokine in culture supernatant, and CBS125149 group up-regulated the protein secretion of TNF-α cytokine obviously.Conclusion The pattern recognition receptors of TLR2, TLR4, Dectin-1 may participate in the recognization of F.monophora by macrophages,and activate the downstream signal molecules MyD88 and NF-κB;CBS122845 can inhibit the expression of TNF-α to maintain long-term growth and reproduction in macrophage.

Key words: Fonsecaea monophora, Toll-like receptor 2, Toll-like receptor 4, Dectin-1, Tumor necrosis factor-α

CLC Number:

R379.9

JIANG Li, ZHANG Jun-Min, SUN Jiu-Feng, XI Li-Yan, CAI Wen-Ying, XIAO Xing. Eeffect of Fonsecaea monophora on the expressions of TLR2,TLR4,Dectin-1 and the production of TNF-α in macrophages[J]. Chinese Journal of Mycology, 2014, 9(3): 134-138.

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