Effects of inhibiting Spitzenkörper formation on proliferation and murine pathogenicity of T. asahii

Abstract

Abstract:

Objective To investigate the effects of proliferation and pathogencity of T.asahii in murine model after inhibititing spitzenkörper formation.Methods The suspension of T.asahii were dealed with different concentration of Cytochalasin D for inhibiting Spitzenkörper formation.In vitro, each suspension was measured in the Growth Curve and colony.In vivo, the different suspensions were inoculated into ten mice through caudal vein respectively, and then the death rate was counted within three weeks. Meanwhile the main viscera of the mice were examined by mycologic culture and pathology and finally the infection rate were counted.Results Compared with the control group,the speed of fungal growth in the experimental group were prolonged about 8~16 h obviously and the colony on culture medium were also minished.The death rate and infection rate of mice were accordingly decreased with the increasing concentration of Cytochalasin D. In other words, pathogencity of T.asahii was clearly cut down.Conclusions When the Spitzenkörper formation being inhibited by Cytochalasin D, the proliferation of T.asahii was restricted and the pathogencity decreased apparently. With the increasing of density of Cytochalasin D, the pathogencity of mice decreased gradiently. It suggested that there might be a clue of a new anti-fungal target.

Key words: Trichosporon asahii, spitzenkö, rper, pathogenicity

CLC Number:

R379.9

LI Mei, WANG Wang-ling, XIA Zhi-kuan, YANG Rong-ya. Effects of inhibiting Spitzenkörper formation on proliferation and murine pathogenicity of T. asahii[J]. Chinese Journal of Mycology, 2014, 9(3): 129-133.

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