Advances in the method of fungi diversity

Abstract

Abstract: Fungi widely exists in nature, playing an important role in the ecosystem. The pervasive application of molecular biology technology in microbial diversity and the emergence of metagenomic breaked the limitations of traditional microbial culture method, so that improve the human's recognition of fungal diversity. This paper reviewed the main development course of studying fungal diversity approaches, introduced several commonly used methods of molecular biology on fungal diversity research, mainly including clone library PCR based, Denaturing gradient gel electrophoresis, Terminal restriction fragment length polymorphism, Real-time fluorescent quantitative PCR, Fluorescence in situ hybridization and barcoded pyrosequencing, and expounded the huge potential of metagenomics in this field.

Key words: fungi, diversity, method

CLC Number:

R379

SHI Li-na, LIU Xiao-feng. Advances in the method of fungi diversity[J]. Chinese Journal of Mycology, 2014, 9(1): 60-64.

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