Construction of knockout strains of MXR1 gene in Candida albicans

Abstract

Abstract: Objective To construct the plasmid for MXR1 disruption, and knockout the MXR1 gene in Candida albicans.Methods Up and down streams of MXR1 gene ORF were amplified and inserted into the p5921 plasmid.MXR1 gene was disrupted according to the Ura-Blaster method. The constructed strains were identified by PCR and Southern-blot methods.Results The knockout plasmid pUC-MXR1-URA3 and MXR1 disruption strains were successfully constructed.Conclusions The MXR1 disruption strains are helpful for the study of the drug-resistance mechanism.

Key words: Candida albicans, MXR1 gene, gene disruption

CLC Number:

R379.4

TAN Hong-yue, LI Ping, HU Chan, ZHU Hong-mei, GU Jun, WEN Hai. Construction of knockout strains of MXR1 gene in Candida albicans[J]. Chinese Journal of Mycology, 2013, 8(2): 69-73.

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