Analysis of the growth characteristics of Aspergillus fumigatus in different temperature and nutrient media

Abstract

Abstract: Objective To determine the growth characteristics of Aspergillus fumigatus in different nutrient media and temperature.Methods Growth curves were used to determine the optimum medium, optimum and maximum growth temperatures for two strains of Aspergillus fumigatus:colony counts were used to determine the fatal temperatures for them.Results Three different nutrient media, yeast extract medium (YG), minimal medium (MM) and yeast nitrogen base (YNB), were tested. YG medium was the optimum medium for measuring the growth curves of Aspergillus fumigatus; In YG medium, the optimum growth temperature for Aspergillus fumigatus was 37℃, and the maximum growth temperature was 52℃. The slower growth rates of strains were observed with gradually elevated temperature. Unexpectedly, the responses of these two strains to high temperature were different:one strain's growth became slower markedly at 48℃ than at 37℃ during log phase, while the other one grew at 48℃ as well as at 37℃ during log phase. The strains of Aspergillus fumigatus were dead when exposed to 65℃ lasting more than 180 min or 70℃ lasting more than 120 min.Conclusions The optimum and the maximum growth temperature for Aspergillus fumigatus were 37℃ and 52℃, respectively.The fatal condition for Aspergillus fumigatus was 65℃ lasting more than 180 min or 70℃ lasting more than 120 min.

Key words: Aspergillus fumigatus, temperature, growth curve

CLC Number:

R379.6

ZHANG Jin-qing, LIU Wei, TAN Jing-wen, WAN Zhe, LI Ruo-yu. Analysis of the growth characteristics of Aspergillus fumigatus in different temperature and nutrient media[J]. Chinese Journal of Mycology, 2013, 8(1): 6-9.

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