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Chinese Journal of Mycology 2012, Vol. 7  Issue (6): 339-341,361.

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Fermentation culture of Cryptococcus neoformans benefiting for purification of mannoprotein

LI Ping, TAN Hong-yue, ZHONG Bin, CHEN Li-hua, HU Chan, ZHU Hong-mei, WEN Hai   

  1. Institute of dermatology and fungal disease,Key Laboratory of the army fungal disease,Department of dermatology,Changzheng Hospital,Shanghai 200003,China
  • Received:2012-07-06 Online:2012-12-28 Published:2012-12-28

Abstract:

Objective To study the possibility of fermentation culture of Cryptococcus neoformans and the advantages of fermentation culture supernatant used for purificating mannoprotein.Methods ConA Sepharose 4B affinity column was used to purificate mannoprotein from fermentation culture supernatant of CAP67.Results Up to milligram of mannoprotein was obtained from fermentation supernatants of Cryptococcus neoformans acapsular strain CAP67.Conclusion Fermentation method can be used to culture Cryptococcus neoformans, and the fermentation supernatants can be used for the purification of mannoprotein,which possessed the superiority of shorter time-consuming and higher yields compared to the traditional method.

Key words: Cryptococcus neoformans, fermentation, capsular polysaccharide, mannoprotein, isolation and purification

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