Detection of (1,3)-β-D-Glucan and Galactomannan for diagnosis of invasive fungal disease

Abstract

Abstract: Objective To evaluate the non-culture diagnostic methods for diagnosis of invasive fungal disease (IFD).Methods A retrospective study was performed on hospitalized patients of Peking University First Hospital who were at risk of IFD. The diagnosis of invasive fungal infection was according to revised definitions. Non-culture methods including galactomannan emzyme immunoassay (GM test)and (1,3)-β-D-glucan assay (G test) were analysed of sensitivity, specificity, and concordance rate, respectively. Two tests were combined to evaluate the changes of sensitivity, specificity, and concordance rate.Results The sensitivity and specificity were 70%, 84% in GM test; 50%, 92% in G test; and 93%, 78% in combined utility of two tests.Conclusions The GM test and G test were both useful in diagnosis of IFD. The better sensitivity and concordance rate might be acquired by combining these two tests.

Key words: IFD, diagnosis, galactomannan,(1,3)-β-D-glucan

CLC Number:

R 446.53

ZHENG Gang, YU Jin, LI Xue-ying, WAN Zhe, LI Ruo-yu. Detection of (1,3)-β-D-Glucan and Galactomannan for diagnosis of invasive fungal disease[J]. Chinese Journal of Mycology, 2012, 7(3): 132-135.

References

[1] Kruger WH,Russmann B,de Wit M,et al. Haemopoirtic cell transplantation of patients with a history of deep or invasive fungal infection during prophylaxis with liposomal amphotericin B[J].Acta Haematol,2005,113(2):104-108.
[2] De Pauw B,Walsh TJ,Donnelly JP,et al.Revised definitions of invasive fungal disease from the European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) Consensus Group[J].Clin Infect Dis,2008,46(12):1813-1821.
[3] Niimi K, Shepherd M G, Cannon R D.Distinguishing Candida species by beta-N-acetyl hexosaminidase activity[J].J Clin Microbiol,2001,39(6):2089-2097.
[4] Aubry A,Porcher R,Bottero J,et.al. Occurrence and kinetics of false-positive Aspergillus galactomannan test results following treatment withβ-lactam antibiotics in patients with hematological disorders [J]. J Clin Microbiol, 2006, 44(2): 389-394.
[5] Mennink-Kersten MA,Donnelly JP,Verweij PE.Detection of circulating galactomannan for the diagnosis and management of invasive aspergillosis[J]. Lancet Infect Dis, 2004, 4(6): 349-357.
[6] Foy PC,van Burik JA,Weisdorf DJ. Galaetomannan antigen enzyme-linked immunosorbent assay for diagnosis of invasive aspergillosis after hematopoietic stem cell transplantation[J].Biol Blood Marrow Transplant, 2007, 13(4): 440-443.
[7] 余进,李若瑜,高露娟,等.血清半乳甘露聚糖和血浆(1,3)β-D-葡聚糖检测诊断侵袭性真菌感染[J].中华医学杂志,2010,90(6):371-374.
[8] Senn L, Robinson JO, Schmidt S, et al. 1,3-Beta-D-glucan antigenemia for early diagnosis of invasive fungal infections in neutropenic patients with acute leukemia[J]. Clin Infect Dis, 2008, 46(6):878-885.
[9] Ostrosky-Zeichner L, Alexander BD, Kett DH, et al. Multicenter clinical evaluation of the (1-->3) beta-D-glucan assay as an aid to diagnosis of fungal infections in humans[J]. Clin Infect Dis, 2005, 41(5):654-659.

[1]	YU Jin, LIU Wei, CHEN Wei, ZHANG Xiao-mei, WAN Zhe, WANG Ai-ping, LI Ruo-yu. Experts opinion on the laboratory diagnosis and treatment of invasive fungal infection secondary to severe novel coronavirus pneumonia [J]. Chinese Journal of Mycology, 2020, 15(1): 1-5.
[2]	SHEN Wang, YANG Wen-li, YEL-yan, TAN X-yu, HUANGC-juan, ZHANG Xin. Clinical value of G test in invasive candidiasis [J]. Chinese Journal of Mycology, 2019, 14(2): 78-82.
[3]	WANG Guang-yu, CHEN Min, HONG Nan, WANG Sheng-qi, LIAO Wan-qing, YANG Ying. Study on the diagnosis of cryptococcal meningoencephalitis by molecular biological techniques [J]. Chinese Journal of Mycology, 2019, 14(2): 95-98.
[4]	ShaoXia, QiaoJian-jun. Tinea incognito on the neck with long-term misdiagnosis [J]. Chinese Journal of Mycology, 2019, 14(1): 45-46.
[5]	HE Yun, ZHENG Hai-ling, LI Xiao-fang, LV Gui-xia, SHEN Yong-nian, LIU Wei-da. A retrospective analysis of phaeohyphomycosis in the Mainland of China (1987-2017) [J]. Chinese Journal of Mycology, 2018, 13(5): 272-276.
[6]	GUO Yan-yang, XUE Xiao-wen, MA Cui-ling, SU Xin, WANG Gang, FU Meng. Nocardiosis with special skin manifestations: a case report with literature reviews [J]. Chinese Journal of Mycology, 2018, 13(5): 283-286.
[7]	HE Xiao-qing, SHEN Yin-zhong. Research progress in the diagnosis and treatment of Pneumocystis pneumonia [J]. Chinese Journal of Mycology, 2018, 13(4): 247-251.
[8]	ZHENG Yan-qing, SHI Na-na, CAO Cun-wei. The research of quantitative real-time PCR testing different specimen in diagnosis of talaromycosis [J]. Chinese Journal of Mycology, 2018, 13(2): 71-74.
[9]	XU Ming-jun, ZHANG Hui-ting, ZHANG Yu-jie, XU Yu-jie, SUN Xiao-peng, MA Xiao-wei. Diagnostic value of plasma 1,3-β-D-glucan combined with Candida score in patients with invasive fungal infections [J]. Chinese Journal of Mycology, 2018, 13(1): 11-14.
[10]	GUO Kai, XIANG Zhen-zhen, SANG Jun-jun, CHEN Li. A misdiagnosed case of invasive aspergillosis [J]. Chinese Journal of Mycology, 2016, 11(6): 372-375.
[11]	XIA Ji-rong, NIU Si-qiang, Cao Ju. Re-evaluation of the clinical value of (1,3)-β-D-glucan detection in the invasive fungal infections [J]. Chinese Journal of Mycology, 2016, 11(5): 269-271,274.
[12]	JI Mei, SHEN Xue-hua. Analysis of 64 cases of tinea facie [J]. Chinese Journal of Mycology, 2016, 11(5): 282-284.
[13]	SHI Xi-wu, XU Jing. Misdiagnosis analysis of a case with facial cutaneous sporotrichosis [J]. Chinese Journal of Mycology, 2016, 11(4): 223-225.
[14]	ZHU Qiang, CHEN Liang-an, YANG Zhen. The reason for cavitating lung adenocarcinoma misdiagnosed as pulmonary aspergillus disease [J]. Chinese Journal of Mycology, 2016, 11(4): 235-239.
[15]	YAN Ru-fan, LI Xin-lei, ZHENG Dong-yan, LIN Bing, LIANG Hao, CAO Cun-wei. The value of the galactomannan antigen assay in diagnosis Penicilliosis marneffei [J]. Chinese Journal of Mycology, 2016, 11(1): 16-19.