Abstract: Objective To investigate the antifungal activity of extracts from Rubus idaeus combined with azole drugs against Candida spp.in vitro.Methods The minimum inhibitory concentration(MIC),the fractional inhibitory concentration index(FICI) of extracts from Rubus idaeus and combination with azole drugs against Candida spp.were determined by M27-A Microdilution Method based on CLSI.Results All extracts from Rubus idaeus by different solution methods showed collaboration with fluconazole.For example,the MIC₈₀ of ethanol extracts from Rubus idaeus against Candida spp.mainly concentrated on 0.16~1.25 mg/mL,while combined with fluconazole showed the collaboration(FICI≤0.5),and the MIC₈₀ reduced to 0.01~0.04 mg/mL.The antifungal effect of fluconazole against Candida spp.was also strengthened.Besides,the MIC₈₀s of ethanol extracts from Rubus idaeus were also reduced from 10 mg/mL to 0.04 mg/mL when being combined with other azole drugs.Conclusion The antifungal effect of ethanol extracts from Rubus idaeus or azole drugs against resistant Candidas was weaker,while the combination showed clear collaboration and the effect against resistant Candida spp.significantly enhanced.

Key words: Rubus idaeus, ethanol extracts, in vitro antifungal activity, fractional inhibitory concentration index(FICI)