Abstract: Objective To investigate the incidence of nosocomial Candida albicans infection in the Drum Town Hospital of Nanjing,and the correlation between drug-resistance and genotyping.Methods We investigated the clinical isolates of Candida albicans from March to November in 2011 retrospectively.Two hundred and thirty-two isolates were selectived randomly for the genotyping,with polymerase chain reaction for the 25S rDNA of Candida albicans.The antifungal susceptibility was carried out with the ATB fungal test.Results The results show that 6196 positive culture strains were isolated in this period,with 973 isolates being fungal strains.The separation rate for Candida albicans was 58%(562/973) and mainly distributed in pneumology department(39.1%),with the main infected sites of lower respiratory tract(81.7),than in urinary tract infection(9.4%).The susceptibility test for the Candida albicans isolates showed that the drug resistance to flucytosine,amphotericin B,fluconazole,itraconazole and voriconazole were 0.9%,0%,1.4%,1.6% and 1.1%,respectively.The 232 clinical isolates were classified into three genotypes: genotype A(125 isolates),genotype B(96 isolates) and genotype C(11 isolates).Antifungal susceptibility test indicated that there were no statistically difference between the genotyping and drug-resistance.Conclusions In conclusion,Candida albicans was the mainly fungal isolate in the Drum Town Hospital of Nanjing,and the majority isolates were from lower respiratory tract.The clinical isolates of Candida albicans showed high susceptibility to the five antifungal drugs above.Genotypes A and B were the mainly isolates of Candida albicans.No statistically difference was found among the five antifungal drugs with the genotypes.

Key words: Candida albicans, genotyping, antifungal susceptibility test