Construction and identification of siRNA expression vector targeting MIS1 gene in Cryptococcus neoformans

Abstract

Abstract: Objective To construct siRNA expression vector targeting MIS1 gene in Cryptococcus neoformans.Methods Genome sequences of MIS1 gene were retrieved from Genebank and siRNA sequences were designed by Ambion online software.The cDNA was synthesized and inserted into Psilencer4.1-CMV neo,then identified through sequencing and real time PCR.Results The recombinant plasmid of siRNA targeting MIS1 in Cryptococcus neoformans completely coincided with the design,and the mRNA level of MIS1 was inhibited significantly.Conclusion The siRNA expression vector was constructed successfully,and laid a foundation for exploring the function of MIS1 in Cryptococcus neoformans.

Key words: Cryptococcus neoformans, siRNA, MIS1

CLC Number:

R379.5

LI Xiu-li, TIAN Yuan, SHI Yu-lin, GU Jun-ying, LI Xiao-jian, LIU Zhi-yu, MA Yue-e, GAO fei, WANG Xiu-xiu. Construction and identification of siRNA expression vector targeting MIS1 gene in Cryptococcus neoformans[J]. Chinese Journal of Mycology, 2012, 7(1): 17-19,23.

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Construction and identification of siRNA expression vector targeting MIS1 gene in Cryptococcus neoformans

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