Rapid clinical yeast identification by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

Chinese Journal of Mycology　2012, Vol. 　Issue (5): 269-272.

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Rapid clinical yeast identification by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

JIN Ying¹, YANG Shuang-feng², WANG Jun-fang¹, LIU Jin¹, CUI Yun-tao¹, ZHOU Xin¹, ZHAO Hua-bing²

Department of laboratory, The Affiliated Hospital of Logistical College of Chinese People's Armed Police Force, Tianjin 300162, China

Received:2012-05-31 Online:2012-10-28 Published:2012-10-28

Abstract

Abstract: Objective To establish a rapid identification method of clinical yeast.Methods Total 150 isolated clinical yeast strains were analyzed by Bruker Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF-MS)and VITEK 2-compact system respectively, and the different results were determined by gene sequencing.Results All 150 clinical yeast strains were identified accurately and rapidly by MALDI-TOF-MS. A total of 100% of isolates were identified correctly at the genus level, with 94% identified correctly at the species level.Conclusion The MALDI-TOF MS-based method was found to be reproducible and accurate, with low consumable costs and minimal preparative time. These results demonstrated that MALDI-TOF-MS could be a rapid technique for identification of clinical yeast.

Key words: MALDI-TOF-MS, clinical yeast, rapid identification

CLC Number:

R446.53

JIN Ying, YANG Shuang-feng, WANG Jun-fang, LIU Jin, CUI Yun-tao, ZHOU Xin, ZHAO Hua-bing. Rapid clinical yeast identification by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry[J]. Chinese Journal of Mycology, 2012, (5): 269-272.

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Rapid clinical yeast identification by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

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