Purification of capsular polysaccharide GXM from Cryptococcus neoformans and it's role in regulating MR expression in macrophages

Abstract

Abstract: Objective To isolate and purificate capsular polysaccharide GXM from Cryptococcus neoformans B3501 and observe whether can GXM regulate MR expression in macrophages.Methods Alcohol was used to precipitate capsular polysaccharide and hexadecyltrimethyl ammonium bromide (CTAB) was used to precipitate GXM specifically. Western blot was used to verify MR expression in macrophages stimulated by GXM for 24 h.Results Up to milligram of GXM was isolated and purificated from the culture supernatant of Cryptococcus neoformans B3501,and MR expression did not change significantly in macrophages stimulated by GXM.Conclusion GXM purificated from Cryptococcus neoformans did not regulate MR expression in macrophages.

Key words: Cryptococcus neoformans, capsular polysaccharide, GXM, isolation and purification, mannose receptor, expression

CLC Number:

R379.5

LI Ping, TAN Hong-yue, HU Chan, CHEN Li-hua, HUANG You-ming, ZHONG Bin, ZHU Hong-mei, WEN Hai. Purification of capsular polysaccharide GXM from Cryptococcus neoformans and it's role in regulating MR expression in macrophages[J]. Chinese Journal of Mycology, 2012, (5): 265-268.

References

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