Abstract: Objective Observe the change of γ-IFN and IL-8 secreted by keratinocytes after Trichophyton rubrum infection, and the effects of TLR2 on γ-IFN and IL-8 secretion. To explore the role of TLR2 in anti-dermatophytic infection.Methods Keratinocytes treated before and after anti-TLR2 monoantibody were stimulated by Trichophyton rubrum.The levels of γ-IFN and IL-8 in supernatant were determined with ELISA at different time points.Results The levels of γ-IFN and IL-8 secreted by kerainocytes were significantly increased after induced with Trichophyton rubrum(P<0.05).The level of γ-IFN reached(85.36±4.54) pg/mL at the point of 4h,and(445.58±13.99) pg/mL at 16 h; After treated with anti-TLR2 monoantibody, the levels of IL-8 were reduced at the points of 2 h,4 h,8 h and 16 h(P<0.05); and the levels of γ-IFN were reduced at the points of 2 h, 4 h and 8 h(P<0.05), but had no statistically significant reduction at the point of 16 h(P>0.05).Conclusion After stimulated keratinocytes,Trichophyton rubrum can promote its secretion of γ-IFN and IL-8; TLR2 play an important role onγ-IFN and IL-8 secretion of keratinocytes.

Key words: keratinocyte, Trichophyton rubrum, TLR2, γ-IFN, IL-8