In vitro activity of terbinafine in combinations with other antifungi drugs against clinical pathogenic fungi of vulvovaginal candidiasis

Abstract

Abstract: Objective To investigate the in vitro activity of terbinafine(TEB)in combinations with, itraconazole(ITC) fluconazole(FLC), clotrimazole(CMZ); miconazole(MCZ) and nystatin(NYS) against clinical isolates of vulvovaginal candidiasis.Methods The minimal inhibitory concentrations(MICs)of TEB, FLU, ITR, MCZ, CMZ and NYS against 46 strains of Candida were determined by using CLSI broth microdilution method(M27-A3).Additionally, the interactions of TEB with ITR,FLU,MCZ, CMZ and NYS, were assessed by using the checkerboard technique. The fractional inhibitory concentration index(FICI) was used to categorize drug interactions as following, synergy, FICI≤0.5; indifference, FICI>0.5 and≤4.0; or antagonism, FICI>4.0.Results The MIC ranges of TEB, FLU, ITR, MCZ, CMZ and NYS against candida. spp were 0.5-256 μg/mL,0.5-128 μg/mL,0.5-32.0 μg/mL,0.125-16 μg/mL,0.03-4.0 μg/mL and 2-8 μg/mL, respectively. The combinations of TEB with FLU, ITR, MCZ, CMZ showed synergic effects are 20/20(100%), 16/20(80%), 11/20(55%)and 18/20(90%)in C.albicans isolates, respectively. Besides, the combinations of TEB with FLU, ITR, MCZ and CMZ showed indifference against Candida krusei,Candida parapsilosis and Candida glabrata. The combinations of TEB with all the azoles showed synergic for Candida guilliermondii. The combinations of TEB with FLU, ITR, MCZ, CMZ showed synergic effects are 67%,60%,20% and 20% in Candida tropicalis, respectively. There was not synergic effect when TEB combined with NYS in all strains.Conclusions Different Candida spp show different effects to the combinations of TEB with azoles. There is not synergic effect when TEB in combination with NYS for all strains.

Key words: terbinafine, drug combinations, Candida spp, vulvovaginal candidiasis

CLC Number:

R379.4

JI Ya-juan, WANG Ai-ping, LIU Wei, WAN Zhe, LI Ruo-yu. In vitro activity of terbinafine in combinations with other antifungi drugs against clinical pathogenic fungi of vulvovaginal candidiasis[J]. Chinese Journal of Mycology, 2012, (2): 73-76,81.

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