Abstract: Objective To explore a practical and effective method for fingi preservation.Methods Seventy-three strains of pathogenic filamentous fungi were preserved on Potato dextrose agar slants at 4℃ and in freezing tubes at-80℃,overlaid with 10%(v/v) glycerol after purification.Results The survival ratioes were 98.6% at -80℃ and 100% at 4℃ after one-year preservation.Conclusions These two methods are simple,convenient,and valueble in preservation of pathogenic filamentous fungi.

Key words: pathogenic fungi, culture collection, filamentous fungi