Abstract: Objective To assess the susceptibilities of Candida species to caspofungin and micafungin.Methods Susceptibilities of Candida isolates to caspofungin,micafungin and fluconazole were determined by microdilution method based on CLSI M27-A2 for consecutive seven days.Results After 48 hours,the medians of MIC₅₀ and MIC₉₀ of C.albicans,C.glabrata,and other Candida spp.to caspofungin were 0.03 and 0.03 μg/mL,0.06 and 0.125 μg/mL,0.125 and 0.5 μg/mL,respectively.The medians of MIC₅₀ and MIC₉₀ of C.albicans,C.glabrata,and other Candida species to micafungin were 0.03 μg/mL and 0.03 μg/mL,0.06 μg/mL and 0.06 μg/mL,0.25 μg/mL and 0.5 μg/mL,respectively.The medians of MIC₈₀ and MIC₁₀₀ of C.albicans,C.glabrata,and other Candida species to fluconazole were 2 μg/mL and 128 μg/mL,64 μg/mL and 128 μg/mL,2 μg/mL and 32 μg/mL,respectively.None of the 85 isolates of Candida species showed cross-resistance to caspofungin,micafungin or fluconazole.To caspofungin,there was no change on MIC₅₀ or MIC₉₀ of C.albicans after 24 hours.No increase on MIC₅₀ and MIC₉₀ of C.glabrata was observed after 72 hours and 120 hours.Neither did increase on MIC₅₀ and MIC₉₀ of other Candida species after 168 hours and 96 hours.To micafungin,there was no change on MIC₅₀ and MIC₉₀ of C.albicans and C.glabrata after 24 hours and no increase on MIC₅₀ and MIC₉₀ of other Candida species after 72 hours.Conclusions Caspofungin and micafungin may have great antifungal activities to all candida species,especially to C.albicans and C.glabrata.The MICs would increase with the exposure time,which show specificity to drug and Candida species.

Key words: dynamic, caspofungin, micafungin, fluconazole, Candida species, in vitro susceptibility