Four methods of DNA extraction for Aspergillus niger

Abstract

Abstract: Objective To find the best DNA extraction method for Aspergillus niger.Methods The purity and output of Aspergillus.niger DNA extracted by benzyl chloride method,CTAB method,Biospin kit and microwave method were compared via agarose gel electrophoresis and PCR.Results The extracted DNA cultured for 3 ds was purer than that for 7-14 ds.All the DNA products were amplifiable for PCR,but the purity and output via CTAB method showed the best results.Conclusions CTAB is suitable for Aspergillus niger DNA extraction.

Key words: fungi, DNA extraction, comparison

CLC Number:

R379.6

ZHANG Xiao-li, LV Xue-lian, SHEN Yong-nian, LV Gui-xia, WANG Miao-miao, GE Yi-ping, LIU Wei-da. Four methods of DNA extraction for Aspergillus niger[J]. Chinese Journal of Mycology, 2011, 6(3): 145-148.

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Four methods of DNA extraction for Aspergillus niger

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