Abstract: Objective To analyze the genotypes of 50 isolates of Candida albicans from Han and Uyghur patients in Xinjiang area and to understand the in vitro antifungal susceptibilities of these isolates against amphotericin B,flucytosine,micafungin,itraconazole,fluconazole and miconazole.Methods The genotypes were identifed by the size of PCR amplicons amplifined from the group I intron contained region of 25S rDNA of C.albicans:genotype A(450 bp),genotype B(840 bp) and genotype C(450 bp and 840 bp).In vitro antifungal susceptibilities of 50 strains to 6 kind of antifungal agents were determined by CLSI M27-A microdilution method.Results Fifty strains were classified into three genotypes,including 30 strains of type A and 10 strains of type B and C,all these strains showed lower MIC to amphotericin B,flucytosine,micafungi,and miconazole(MIC range:0.25-0.5 μg/mL,0.125-0.25 μg/mL,≤0.03 μg/mL,and 0.25-8 μg/mL,respectively),and higher MIC to itraconazole and fluconazole(MIC range:0.25-8 μg/mL and 0.5-64 μg/mL,respectively).The MIC of the genotype B and C strains to flucytosine was 0.125 μg/mL.The resistant ratio of itraconazole and fluconazole were 84% and 70%,respectively.No statistical significance was found in the genotypes of the strains from different races(P>0.05) or different genotypic strains and antigungal susceptibilities(P>0.05).Conclusions C.albicans in Xinjiang area included 3 genotypes(A,B and C).There was no correlation between the genotype and antifungal susceptibilities of C.albicans strains from Han and Uyghur patients.Nither did genotype and race.All strains were resistant to itraconazole and flucytosine,but genotype B and C showed high susceptibilities to flucytosine.

Key words: Xinjiang area, Candida albicans, genotypes, in vitro antifungal susceptibilities