PCR identification of Trichophyton rubrum

Abstract

Abstract: Obiective To develop a rapid method to for identification of Trichophyton rubrum.Methods Primed ITS1986 5’GAC ACC AAG AAA AAA TTC TCT GAA GA3’,ITS2441 5’GTC CTG AGG GCG CTG AA3’ were chosen to amplify 45 strains of Trichophyton rubrum,5 strains of Trichophyton mentagrophytes and 1 strain of Trichophyton violaceum.Results Characteristic bands were amplified in Trichophyton rubrum.But there was no characteristic band found in Trichophyton mentagrophytes and Trichophyton violaceum.Conclusions Trichophyton rubrum could be identificated by PCR rapidly.

Key words: Trichophyton rubrum, PCR, Trichophyton mentagrophytes, dermatophytes

CLC Number:

R379.2

FEI Shi, GAO Ai-li, LI Xiao-dong, LI Zhen-jie, XIAO Chang-qing, ZHU Hui-lan. PCR identification of Trichophyton rubrum[J]. Chinese Journal of Mycology, 2010, 5(5): 282-285.

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