Antifungal activity of fluconazole against Ssk1p mutant of Candida albicans

Abstract

Abstract: Objective To investigate the in vitro antifungal activity of fluconazole against two-component signal transduction protein Ssk1p mutant of Candida albicans (SSK21).Methods The minimum inhibitory concentrations (MIC) of fluconazole against SSK21 and wild type of C.albicans (CAF2-1) were tested by broth microdilution and ergosterol content assays.RT-PCR was performed to determine the expression of SSK1 in CAF2-1 in the presence of fluconazole.Results The MIC of fluconazole against SSK21 was 0.032 μg/mL,while that against CAF2-1 was 16 μg/mL.After administration with fluconazole,the transcription level of SSK1 from CAF2-1 was increased.Conclusions SSK21 is hypersensitive to fluconazole compared with wild type strain CAF2-1.Ssk1p and related genes should be exploited for new therapeutic approach.

Key words: Candida albicans, two-component signal transduction, fluconazole, drug sensitivity

CLC Number:

R379.4

LI Xiao-fang, Neeraj Chauhan, Richard Calderone, LIU Wei-da. Antifungal activity of fluconazole against Ssk1p mutant of Candida albicans[J]. Chinese Journal of Mycology, 2010, 5(5): 269-272.

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