Phenotype switching of Candida parapsilosis

Abstract

Abstract: Objective To investigate the phenotype switching of Candida parapsilosis on different medium and the effect of temperature.Methods Nine isolates of C.parapsilosis collected from the skin of healthy people and clinic patients were vauinated on modified Lee’s medium and YPD medium supplemented with phloxine B.Phenotype switching and the effect of different temperature were studied.Results High-frequency phenotypic switching and white opaque switch of C.parapsilosis were observed on both Lee’s medium and YPD medium supplemented with phloxine B.The proportion of opaque colony under 37℃ was lower than that under 25℃ on Lee’s medium.Conclusions C.parapsilosis is capable of high-frequency phenotypic switching and white-opaque transition with stable smooth form at 37℃.

Key words: Candida parapsilosis, phenotype switching, white, opaque

CLC Number:

R379.4

WANG Miao-miao, LV Xue-lian, LIU Ze-hu, LV Xue-lian, SHEN Yong-nian, LIU Wei-da. Phenotype switching of Candida parapsilosis[J]. Chinese Journal of Mycology, 2010, 5(2): 87-91.

References

[1] Slutsky B,Staebell M,Anderson J,et al."White-opaque transition":a second high-frequency switching system in Candida albicans[J].J Bacteriol,1987,169 (1):189-197.
[2] Kavanagh K.Medical mycology:cellular and molecular techniques[M].New York:John Wiley & Sons,Ltd,2007.
[3] Pfaller MA,Diekema DJ.Epidemiology of invasive candidiasis:a persistent public health problem[J].Clin Microbiol Rev,2007,20(1):133-163.
[4] Mokaddas EM,Al-Sweih NA,Khan ZU.Species distribution and antifungal susceptibility of Candida bloodstream isolates in Ku-wait:a 10-year study[J].J Med Microbiol,2007,56 (Pt 2):255-259.
[5] Soll DR.High-frequency switching in Candida albicans[J].Clin Microbiol Rev,1992,5 (2):183-203.
[6] Laffey SF,Butler G.Phenotype switching affects biofilm forma-tion by Candida parapsilosis[J].Microbiology,2005,151 (Pt 4):1073-1081.
[7] Lockhart SR,Pujol C,Daniels K J,et al.In Candida albicans,white-opaque switchers are homozygous for mating type[J].Genetics,2002,162 (2):737-745.
[8] Miller MG,Johnson AD.White-opaque switching in Cartdida albicans is controlled by mating-type locus homeodomain proteins and allows efficient mating[J].Cell,2002,110(3):293-302.
[9] Enger L,Joly S,Pujol C,et al.Cloning and characterization of a complex DNA fingerprinting probe for Candida parapsilosis[J].J Clin Microbiol,2001,39(2):658-4569.
[10] Logue ME,Wong S,Wolfe KH,et al.A genome sequence survey shows that the pathogenic yeast Candida parapsilosis has a defective MTLa1 allele at its mating type locus[J].Eukaryot Cell,2005,4(6):1009-1017.
[11] Lachke SA,Lockhart SR,Daniels KJ,et al.Skin facilitates Candida albicans mating[J].Infect Immun,2003,71 (9):4970-4976.
[12] Kvaal CA,Srikantha T,Soll DR.Misexpression of the whitephase-specific gene WH11 in the opaque phase of Candida albicans affects switching and virulence[J].Infect Immun,1997,65(11):4468-4475.
[13] Miller NS,Dick JD,Merz WG.Phenotypic switching in Candida lusitaniae on copper sulfate indicator agar:association with amphotericin B resistance and filamentation[J].J Clin Microbiol,2006,44 (4):1536-1539.
[14] Lachke SA,Joly S,Daniels K,et al,Phenotypic switching and filamentation in Candida glabrata[J].Microbiol,2002,148(Pt 9):2661-2674.