Identification of prototheca by phenotype and molecular biology techniques

Abstract

Abstract: Objective To identify Prototheca and its varieties by phenotype and molecular biology techniques.Methods The structure and phenotypic characteristics, physiology, biochemistry and molecular biological identification, sensitivity to common anti-fungal drugs and characteristics of histopathology were reviewed,summarized and analyzed.Methods Two strains of Prototheca (P.zopfii var.hydrocarbonea and P.zopfii var .portoricensis) were identified from cerebrospinal fluid and lymph nodes by phenotypic and molecular biology techniques and relevent photographs were showed.Conclusions Diagnosis of Protothecosis mainly depends on mycological examinations including direct microscopic examination,culture,and histopathology because of no characteristic in clinical.Carbohydrates assimilation, temperature resistance and molecular biological methods would be helpful for strain identification.

Key words: P.zopfii var.hydrocarbonea, P.zopfii var.portoricensis, identification, molecular biological identification

CLC Number:

R756

ZHANG Qiang-qiang, ZHAO Ying. Identification of prototheca by phenotype and molecular biology techniques[J]. Chinese Journal of Mycology, 2010, 5(1): 9-12,25.

References

[1] Lass-Florl C,Mayr A.Human protothecosis[J]. Clin Microbiol Rev,2007,20(2):230-242.
[2] Lee JS,Moon GH,Lee NY,et al.Case report:protothecal te-nosynovitis[J]. Clin Orthop Res,2008,466(12):3143-3146.
[3] Lanotte P,Baty G,Senecal D,et al.Fatal algaemia in patient with chronic lymphocytic leukemia[J]. Emerg Infect Dis,2009,15(7):1129-1130.
[4] Narita M,Muder RR,Cacciareili TV,et al.Protothecosis after live transplantation[J]. Liver Transplantation,2008,14 (8):1211-1215.
[5] Zhang QQ,Weng XH,Li L,et al.An unusual case of granulo-matous lymphadenitis due to P.zopfii vat.portoricensis in an im-munocompetent man in China[J]. Int J Infect Dis,2010,Jan 29[Epub ahead of print].
[6] 吕桂霞,沈永年,吴绍熙,等.面部皮肤型无绿藻病国内首例报告[J]. 中华皮肤科杂志,1994,27(3):139-140.
[7] 张金松,刘志梅,张信江,等.皮肤中型原藻病[J]. 临床皮肤科杂志,2006,35(11):705-706.
[8] 章强强,翁心华,朱利平,等.小型无绿藻性脑膜炎及其真菌学研究[J]. 中华皮肤科杂志,2006,39(8):445-447.
[9] 廖康,陈冬梅,罗兰,等.脑脊液分离威克原膜菌1例[J]. 中华检验医学杂志,2003,26(3):180-181.
[10] 官裕宗,李育佳.皮肤型无绿藻病台湾首例报告[J]. 台湾医志,1989,88:747-751.
[11] 吴绍熙,吕桂霞,姜祎群,等.无绿藻病[J]. 临床皮肤科杂志,2007,36(2):127-128.
[12] Roesler U,Moiler A,Hansel A,et al.Diversity within the cur-rent algal species Prototheca zopfii:a proposal for two Prototheca zopfii genotypes and description of a novel species,Prototheca blaschkeae sp.Nov[J]. Int J Syst Evol Microbiol,2006,56(Pt 6):1419-1425.
[13] Ueno R,Hanagata N,Urano N,et al.Molecular phylogany and phenotypic variation in the heterotrophic green algal genus Prototheca[J]. J Phycol,2005,41:1268-1280.
[14] Iacoviello VR,DeGirolami PC,Lucarini J,et al.Protothecosis complicating prolonged endotracheal intubation:case report and literature review[J]. Clin Infect Dis,1992,15(6):959-967.
[15] 刘素玲,冉玉平,曾蔚,等.一种适用于PCR反应的酵母菌、无绿藻及丝状真菌DNA提取方法[J]. 中国真菌学杂志,2006,1(6):340-342.
[16] 刘素玲,冉玉平,何晓丹,等.采用rDNA测序的方法鉴定1株祖菲无绿藻碳水化合物变种[J]. 中国人兽共患病学报,2007,23(7):656-660.
[17] Kurtzman CP,Robnett CJ.Identification and phylogeny of asco-mycetous yeasts from analysis of nuclear large subunit (26S) ri-bosomal DNA partial sequences[J]. Antonie van Leeuwenhoek,1998,73(4),331-371.
[18] Leaw SN,Chang HC,Sun HF,et al.Identification of medically important yeast species by sequence analysis of the internal tran-scribed spacer regions[J]. J Clin Microbiol,2006,44(3):693-699.
[19] Roesler U,Scholz H,Hensel A.Emended phenotypic character-ization of Prototheca zopfii:a proposal for three biotypes and standards for their identification[J]. Int J Syst Evol Microbiol,2003,53(Pt 4):1195-1199.
[20] Ueno R,Urano N,Suzuki M.Phylogeny of the non-photosyn-thetic green micro-algal genus Prototheca (Trebonxiaphyceae,Chlorophyta) and related taxa inferred from SSU and LSU riboso-mal DNA partial sequence data[J]. FEMS Microbiol Lett,2003,223(2):275-280.
[21] Linares MJ,Solis F,Casal M.in vitro activity of voriconazole a-gainst Prototheca wickerhamii:comparative evaluation of sensiti-tre and NCCLS M27-A2 methods of detection[J]. Clin Microbi-ol,2005,43:2520-2522.
[22] Tyring S,Lee P,Walsh P,et al.Papular protothecosis of the chest.Immunologic evaluation and treatment with a combination of oral tetracycline and topical amphotericin B[J]. Arch Derma-tol,1989,125:1249-1252.
[23] 燕勇,李卫平,高雯洁,等.rDNA-ITS序列分析在真菌鉴定中的应用[J]. 中国卫生检验杂志,2008,18(10):1958-1961.