钙调磷酸酯酶Calcineurin在控制马尔尼菲篮状菌无性发育、极性生长、压力应激中的作用研究

中国真菌学杂志　 2022, Vol. 17 　Issue (2): 137-145.

钙调磷酸酯酶Calcineurin在控制马尔尼菲篮状菌无性发育、极性生长、压力应激中的作用研究

黄啟华^1,2,3, 罗宏⁵, 潘开素^1,2,3, 廖柳维^1,2,3, 郑艳青^2,3,4, 曹存巍^1,2,3

1. 广西医科大学第一附属医院皮肤性病科, 南宁 530021;
2. 广西卫生健康委员会真菌研究与真菌病防治重点实验室, 南宁 530021;
3. 广西艾滋病防治研究重点实验室, 南宁 530021;
4. 南宁市第四人民医院, 南宁 530023;
5. 长沙市第一人民医院皮肤性病科, 长沙 410005

收稿日期:2021-10-29 出版日期:2022-04-28 发布日期:2022-05-07
通讯作者: 曹存巍,E-mail:caocunwei@yeah.net E-mail:caocunwei@yeah.net
作者简介:黄啟华,男(汉族),硕士研究生在读.E-mail:599063360@qq.com;罗宏,男(汉族),博士,主任医师.E-mail:lhdoctor@yeah.net
基金资助:
广西自然科学基金（2018GXNSFAA294090）；广西亚热带真菌与真菌病研究重点实验项目（YYZS2020006）；广西研究生教育创新计划项目（YCSW2021123）

The effects of calcineurin on asexual development, polarity and stress responses in Talaromyces marneffei

HUANG Qihua^1,2,3, LUO Hong⁵, PAN Kaisu^1,2,3, LIAO Liuwei^1,2,3, ZHENG Yanqing^2,3,4, CAO Cunwei^1,2,3

1. Department of Dermatology and Venereology, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China;
2. Guangxi Health Commission Key Lab of Fungi and Mycosis Research and Prevention, Nanning, China;
3. Guangxi Key Laboratory of AIDS Prevention and Treatment, Nanning 530021, China;
4. The Fourth People's Hospital of Nanning, Nanning 530023, China;
5. The First Hospital of Changsha, Changsha 410005, China

Received:2021-10-29 Online:2022-04-28 Published:2022-05-07

摘要/Abstract

摘要： 目的明确钙调磷酸酯酶calcineurin调节亚基编码基因（cnaB）及催化亚基编码基因（cnaA）在马尔尼菲篮状菌无性发育、极性生长、压力应激的作用。方法与母本FRR2161（wild-type）对比，观察cnaB基因敲除株（ΔcnaB）、cnaA基因敲除株（ΔcnaA）在25℃、37℃的生长、形态发生及压力应激变化。结果 ΔcnaB敲除株、ΔcnaA敲除株在无性发育、极性生长、压力应激均存在严重缺陷;ΔcnaA在细胞溶解分离、压力应激表现出更严重缺陷。结论 cnaB、cnaA对马尔尼菲篮状菌无性发育、极性生长、压力应激均发挥着重要功能;但cnaA在细胞溶解分离、氧化应激、盐应激等表现出更为重要的调控作用。

关键词: 马尔尼菲篮状菌, 钙调磷酸酯酶, 调节亚基, 催化亚基, 无性发育, 极性生长, 压力应激

Abstract: Objective To determine the functions of calcineurin B (cnaB) and meanwhile further investigate the effects of calcineurin A (cnaA) from Talaromyces marneffei on asexual development, polarity and stress responses. Methods The growth, morphogenesis and external stress responses of strains deleted for cnaB (ΔcnaB strain) or CnaA (ΔcnaA strain) were observed and compared with those of parental strain FRR2161 (wild-type) at both 25℃ and 37℃. Results Both ΔcnaB and ΔcnaA gained severe defects in asexual development, polarity and stress responses. ΔcnaA strain exhibited more severe defects in cellular lysis, separation and resistance to diverse stressors. Conclusion Both cnaB and cnaA exert a key role on Talaromyces marneffei asexual development, polarity and stress responses, but cnaA seems to play a major role in some biological procedures like cellular separation, oxidative stress and salt stress.

Key words: Talaromyces marneffei, calcineurin, regulatory subunit, catalytic subunit, asexual development, polarity, stress response

中图分类号:

R379.9

黄啟华, 罗宏, 潘开素, 廖柳维, 郑艳青, 曹存巍. 钙调磷酸酯酶Calcineurin在控制马尔尼菲篮状菌无性发育、极性生长、压力应激中的作用研究[J]. 中国真菌学杂志, 2022, 17(2): 137-145.

HUANG Qihua, LUO Hong, PAN Kaisu, LIAO Liuwei, ZHENG Yanqing, CAO Cunwei. The effects of calcineurin on asexual development, polarity and stress responses in Talaromyces marneffei[J]. Chinese Journal of Mycology, 2022, 17(2): 137-145.

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