MADLI-TOF MS自建库在马尔尼菲篮状菌快速鉴定中的应用

中国真菌学杂志　 2021, Vol. 16 　Issue (4): 229-233.

MADLI-TOF MS自建库在马尔尼菲篮状菌快速鉴定中的应用

郭鹏豪¹, 伍众文¹, 邱丹萍², 谢梅英³, 刘敏¹, 廖康¹

1. 中山大学附属第一医院检验科, 广州 520080;
2. 广西玉林市人民医院检验科, 玉林 537000;
3. 广西防城港市人民医院检验科, 防城港 538000

收稿日期:2020-05-28 发布日期:2021-08-31
通讯作者: 廖康,E-mail:liaokang1971@163.com E-mail:liaokang1971@163.com
作者简介:郭鹏豪,男(汉族),本科,主管技师.E-mail:jackgph@163.com

Application of MADLI-TOF MS in rapid identification of Talaromyces marneffei

GUO Penghao¹, WU Zhongwen¹, QIU Danping², XIE Meiying³, LIU Min¹, LIAO Kang¹

1. Department of Clinical Microbiology Laboratory, First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, China;
2. Department of Clinical Microbiology Laboratory, Yulin First People's Hospital, Yulin 537000, China;
3. Department of Clinical Microbiology Laboratory, Fang Chenggang First People's Hospital, Fang Chenggang 538000, China

Received:2020-05-28 Published:2021-08-31

摘要/Abstract

摘要： 目的评价基质辅助激光解吸电离飞行时间质谱（MADLI-TOF MS）自建库对临床分离马尔尼菲篮状菌快速鉴定的能力。方法收集广州、玉林、防城港3个地区临床分离的马尔尼菲篮状菌，通过ITS区扩增测序进行菌种准确鉴定。菌株通过微型玻璃珠研磨结合甲酸/乙腈提取法预处理。通过采集建库菌株不同时间点、不同平板上菌丝相和酵母相菌落的质谱数据，建立包含马尔尼菲篮状菌超级图谱的自建库；并用非建库的马尔尼菲篮状菌及临床常见的真菌评估建立的自建库。结果建立了包含具有39个特征峰的马尔尼菲篮状菌超级图谱的自建库；用于评估的菌株均被鉴定到种，准确率为100%。结论 MADLI-TOF MS建立的马尔尼菲篮状菌自建库能够对于临床马尔尼菲篮状菌进行快速、准确的鉴定。

关键词: 马尔尼菲篮状菌, MADLI-TOF MS, Vitek MS

Abstract: Objective Evaluation of the application of MADLI-TOF MS in rapid identification of T. marneffei. Methods T. marneffei isolates were collected from Guangzhou, Yulin and Fangchenggang. All the isolates identified by ITS region amplification and sequencing. The strains were pretreated by glass bead grinding and formic acid/acetonitrile extraction. The spectra of the mold and yeast phase colonies of the establishment strains on different plates and at different time points were obtained. The collected spectra were used for the establishment of the super-spectrum. The remaining isolates and other common clinical fungi isolates were used to evaluate the self-established database. Results The super-spectrum of T. marneffei with 39 characteristic peaks was established. The RUO SARAMIS database was expanded. All the validation strains were correctly identified by the expanded database, with an accuracy of 100%. Conclusion MADLI-TOF MS can quickly and accurately identify T. marneffei by the expanded database.

Key words: Talaromyces marneffei, MADLI-TOF MS, Vitek MS

中图分类号:

郭鹏豪, 伍众文, 邱丹萍, 谢梅英, 刘敏, 廖康. MADLI-TOF MS自建库在马尔尼菲篮状菌快速鉴定中的应用[J]. 中国真菌学杂志, 2021, 16(4): 229-233.

GUO Penghao, WU Zhongwen, QIU Danping, XIE Meiying, LIU Min, LIAO Kang. Application of MADLI-TOF MS in rapid identification of Talaromyces marneffei[J]. Chinese Journal of Mycology, 2021, 16(4): 229-233.

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