142例支气管肺泡灌洗液真菌免疫荧光染色检测结果与G/GM试验结果对比分析

中国真菌学杂志　 2021, Vol. 16 　Issue (1): 6-9.

142例支气管肺泡灌洗液真菌免疫荧光染色检测结果与G/GM试验结果对比分析

李刚荣¹, 白明明², 陶朝欣², 杨会涛², 许丽亚²

1. 河北省强制隔离戒毒所医院检验科, 石家庄 050081;
2. 石家庄荣瑷医学检验所检验科, 石家庄 050021

收稿日期:2020-01-05 发布日期:2021-02-25
作者简介:李刚荣,男(汉族),本科,主管技师.E-mail:369584507@qq.com

A comparative analysis of fluorescent staining and G/GM test of bronchoalveolar lavage fluid in 142 cases

LI Gangrong¹, BAI Mingming², TAO Chaoxin², YANG Huitao², XU Liya²

1. Department of laboratory, Hebei compulsory isolation and rehabilitation hospital, Shijiazhuang 050081, China;
2. Department of laboratory, Shijiazhuang Rongai medical laboratory, Shijiazhuang 050021, China

Received:2020-01-05 Published:2021-02-25

摘要/Abstract

摘要： 目的探讨真菌免疫荧光染色检测支气管肺泡灌洗液（brondioalveolar lavage fluid，BALF）在肺部真菌感染诊断中的意义。方法对2019年1月至12月送检至本所的142例BALF标本进行G/GM试验及真菌免疫荧光染色检测。结果 142例BALF中免疫荧光染色检测阳性为52例（占36.62%），G试验同时阳性者为49例，GM试验同时阳性者为11例。免疫荧光染色检测阳性例数与KOH湿片镜检阳性27例（占19.01%）比较检出率明显提高。结论真菌免疫荧光染色检测作为一种快速方便高效直观的真菌常规检测方法，是BALF标本中真菌检测的1个重要补充，为临床肺部真菌感染的诊断提供了1种快速检测方法。

关键词: 灌洗液, 免疫荧光染色, (1,3)-β-D葡聚糖试验, 半乳甘露聚糖抗原检测试验

Abstract: Objective To investigate the significance of detection of bronchoalveolar lavage fluid (BALF) by fungal immunofluorescent staining in the diagnosis of pulmonary fungal infection. Methods From January to December 2019, 142 BALF samples sent to our institute were tested by G/GM test and fungal immunofluorescent staining. Results In BALF of 142 cases,52 cases (36.62%) were positive by immunofluorescent staining, 49 cases were positive by G test, and 11 cases were positive by GM test. The positive rate of immunofluorescent staining was significantly higher than that of KOH microscopic examination in 27 cases (19.01%). Conclusion As a fast, convenient, efficient and direct routine detection method of fungi, direct microscopy with immunofluorescent staining was an important supplement for the detection of fungi in lavage fluid samples, and provided a fast detection method for the diagnosis of clinical pulmonary fungal infection.

Key words: lavage solution, fluorescent staining, (1,3)-β-D glucan test, galactomannan antigen test

中图分类号:

R446.5

李刚荣, 白明明, 陶朝欣, 杨会涛, 许丽亚. 142例支气管肺泡灌洗液真菌免疫荧光染色检测结果与G/GM试验结果对比分析[J]. 中国真菌学杂志, 2021, 16(1): 6-9.

LI Gangrong, BAI Mingming, TAO Chaoxin, YANG Huitao, XU Liya. A comparative analysis of fluorescent staining and G/GM test of bronchoalveolar lavage fluid in 142 cases[J]. Chinese Journal of Mycology, 2021, 16(1): 6-9.

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