酵母相和菌丝相白念珠菌感染阴道上皮细胞免疫反应的比较

中国真菌学杂志　 2019, Vol. 14 　Issue (4): 193-199.

论著下一篇

酵母相和菌丝相白念珠菌感染阴道上皮细胞免疫反应的比较

王钰婷¹, 王玉柱², 刘锦燕³, 孟玲宁¹, 李卫华², 项明洁^1,3

1. 上海交通大学医学院附属瑞金医院检验科, 上海 200025;
2. 复旦大学生殖与发育研究院上海市计划生育科学研究所, 国家卫生健康委员会计划生育药具重点实验室, 上海 200032;
3. 上海交通大学医学院附属瑞金医院卢湾分院放免检验科, 上海 200020

收稿日期:2019-01-22 出版日期:2019-08-28 发布日期:2019-08-28
通讯作者: 项明洁,E-mail:mjxiang123456@126.com;李卫华,E-mail:iamliwehua@yahoo.com E-mail:mjxiang123456@126.com;iamliwehua@yahoo.com
作者简介:王钰婷,女(汉族),硕士研究生在读.E-mail:yutingwang95@163.com
基金资助:
国家自然科学基金面上项目（81871706），上海市卫计委课题（201840227，201740069），上海市黄浦区卫计委课题（HKM201702）

Comparison of immune responses of yeast form and hyphae form Candida albicans to infection of vaginal epithelial cells

WANG Yu-ting¹, WANG Yu-zhu², LIU Jin-yan³, MENG Ling-ning¹, LI Wei-hua², XIANG Ming-jie^1,3

1. Department of Clinical Laboratory, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China;
2. State Key Labs of Family Planning Devices, National Health Commission of the People's Republic of China, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China;
3. Radioimmunology and Clinical Laboratory, Luwan Branch, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200020, China

Received:2019-01-22 Online:2019-08-28 Published:2019-08-28

摘要/Abstract

摘要：

目的白念珠菌作为一种二相菌，存在酵母相和菌丝相二态，本实验研究这两种不同形态的白念珠菌刺激阴道上皮细胞，比较其引起的免疫应答差异。方法以阴道上皮细胞株VK2/E6E7为模型，分别给予不同浓度的活的白念珠菌和热灭活白念珠菌SC5314（感染指数MOI分别为10、50、100）进行刺激，以实时荧光定量PCR（RT-qPCR）和酶联免疫吸附法（ELISA）检测3 h、6 h、12 h感染组细胞的促炎因子TNF-α和趋化因子IL-8的分泌水平。结果活的白念珠菌在K-SFM细胞培养基中呈菌丝相，热灭活的白念珠菌在K-SFM培养基中呈酵母相。两相感染组分泌IL-8和TNF-α水平与未感染组相比具有显著差异。在各个MOI条件下，菌丝相感染组释放细胞因子mRNA水平均显著高于酵母相；在MOI=10时，菌丝相感染组释放细胞因子的蛋白水平强于酵母相感染组，而随着MOI的增大，结果相反。结论酵母相和菌丝相白念珠菌均能诱发阴道上皮细胞出现显著免疫反应。同时，在MOI=10时，菌丝相白念引起的免疫反应强于酵母相白念。两相感染组免疫反应强度的差异提示两者引起免疫应答的机制可能不同。

关键词: 白念珠菌, 外阴阴道念珠菌病, 菌丝, 阴道上皮, 炎症

Abstract:

Objective Candida albicans has morphological plasticity and can switch morphology among yeast form and hyphal form. In this study, the two different forms of Candida albicans stimulate the vaginal epithelial cells in order to compare the differences in immune response caused by them. Methods Vaginal epithelial cell lines VK2/E6E7 were treated as model. Different concentrations of activated Candida albicans and heat-inactivated Candida albicans SC5314 (MOI=10,50,100) were given to in vitro stimulate vaginal epithelial cell lines. Real-time fluorescent quantitative PCR (RT-qPCR) and enzyme-linked immunosorbent assay (ELISA) method were used to determine the expression of proinflammatory factor TNF-α and chemokines IL-8 under different infection stage(3 h,6 h,12 h). Results Activated Candida alibcans showed hyphal form in K-SFM medium while the heat-inactivated one showed yeast form. Two infection groups showed difference in expression of IL-8 and TNF-α compared to the uninfected group significantly. Under various MOI conditions, the mRNA level of[JP+3]cytokines released by hyphal form infection group was significantly higher than that of yeast form. In especial, at the concentration of MOI=10, the protein level of cytokines released by hyphal form was higher than that of the yeast form. However, the condition turned to reversion with the increase of MOI. Conclusion Both yeast and hyphal form of Candida albicans can induce significant immune responses in vaginal epithelial cells. What's more, hyphal form can induce the immune response of vaginal epithelial cells stronger than that of yeast form under MOI=10. The difference in the immune response intensity by the two-form infection group suggested that the mechanisms of the immunity might be different between the two groups.

Key words: Candida albicans, vulvovaginal vandidiasis, hyphae, vaginal epithelium, inflammation

中图分类号:

R519.3

王钰婷, 王玉柱, 刘锦燕, 孟玲宁, 李卫华, 项明洁. 酵母相和菌丝相白念珠菌感染阴道上皮细胞免疫反应的比较[J]. 中国真菌学杂志, 2019, 14(4): 193-199.

WANG Yu-ting, WANG Yu-zhu, LIU Jin-yan, MENG Ling-ning, LI Wei-hua, XIANG Ming-jie. Comparison of immune responses of yeast form and hyphae form Candida albicans to infection of vaginal epithelial cells[J]. Chinese Journal of Mycology, 2019, 14(4): 193-199.

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酵母相和菌丝相白念珠菌感染阴道上皮细胞免疫反应的比较

Comparison of immune responses of yeast form and hyphae form Candida albicans to infection of vaginal epithelial cells

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