5-氨基酮戊酸光动力疗法体外抑制断发毛癣菌肉芽肿株增殖研究

中国真菌学杂志　 2018, Vol. 13 　Issue (3): 139-143.

5-氨基酮戊酸光动力疗法体外抑制断发毛癣菌肉芽肿株增殖研究

吴倩倩¹, 石磊², 张海艳², 张玲琳², 高志琴², 杨虹², 杨连娟², 王秀丽^1,2

1. 安徽医科大学上海皮肤病临床学院, 上海 200443;
2. 上海市皮肤病医院同济大学医学院, 上海 200443

收稿日期:2018-02-28 出版日期:2018-06-28 发布日期:2018-06-28
通讯作者: 王秀丽,E-mail:wangxiuli_1400023@tongji.edu.cn E-mail:wangxiuli_1400023@tongji.edu.cn
作者简介:吴倩倩,女(汉族),硕士研究生在读.E-mail:wuqian3224@163.com
基金资助:
国家自然科学基金（81601601，81472538）

In vitro study of the 5-aminolevulinic acid photodynamic therapy for Trichophyton tonsurans strain isolated from granuloma

WU Qian-qian¹, SHI Lei², ZHANG Hai-yan², ZHANG Ling-lin², GAO Zhi-qin², YANG Hong², YANG Lian-juan², WANG Xiu-li^1,2

1. Shanghai Clinical College of Skin Disease, Anhui Medical University, Shanghai 200443, China;
2. Shanghai Skin Disease Hospital, Tongji University School of Medicine, Shanghai 200443

Received:2018-02-28 Online:2018-06-28 Published:2018-06-28

摘要/Abstract

摘要：

目的探讨5-氨基酮戊酸光动力疗法（5-Aminolevulinic acid photodynamic therapy，ALA-PDT）对体外培养的断发毛癣菌肉芽肿株的杀伤效应。方法将临床Majocchi肉芽肿患者皮损分离培养出的断发毛癣菌肉芽肿株，在马铃薯葡萄糖琼脂培养基上活化后制备菌悬液，与5 mmol/L ALA共孵育后荧光显微镜观察原卟啉PpⅨ（ProtoporphyrinⅨ，PpⅨ）产生情况。实验分对照组、单药组、单光组和ALA-PDT组。对照组无处理。ALA-PDT组断发毛癣菌与5 mmol/L ALA共孵育6 h后，采用功率密度为40 mW/cm²的633 nm红光以不同能量密度（50、100、150、175、200 J/cm²）照射。单药组断发毛癣菌与5 mmol/L ALA共孵育6 h，不照光。单光组无ALA共孵育，只给予200 J/cm²红光照射。通过数码相机拍摄照片及菌浓度评估ALA-PDT对断发毛癣菌肉芽肿株生长的抑制效应。结果荧光显微镜下可见明显砖红色荧光物质PpⅨ产生，当照光能量密度为175、200 J/cm²时，照片显示菌落生长明显受限，数量减少，菌浓度计数分别为（0.53±0.058）×10⁵ CFU/mL、（0.13±0.057）×10⁵ CFU/mL，明显低于对照组、单药组、单光组及其他低能量密度ALA-PDT组（P<0.05）。结论 ALA-PDT对断发毛癣菌肉芽肿株有明确的杀伤效应，可为临床ALA-PDT治疗皮肤癣菌感染提供理论依据。

关键词: 光动力治疗, 断发毛癣菌, Majocchi肉芽肿, 皮肤癣菌

Abstract:

Objective To demonstrate the fungicidal effect and action mechanism of 5-aminolevulinic acid photodynamic therapy (ALA-PDT) against Trichophyton tonsurans strain isolated from granuloma. Methods The clinical isolate of Trichophyton tonsurans granuloma was cultured in potato dextrose agar medium to prepare the suspension. After 6 hours of incubation, the production of PpⅨ was observed by fluorescence microscopy. Following PDT (at light energy density of 50, 100, 150, 175, or 200 J/cm²), the growth inhibitory of this T.tonsurans strain was observed by colony cultivation. Results Fluorescence microscopy showed obvious PpⅨ production of brick red fluorescent substance. At the light energy density of 175 and 200 J/cm², the significantly restricted growth of colonies was evidenced by decreased concentration of suspension. The suspension concentrations were (0.53±0.058)×10⁵ CFU/mL and (0.13±0.057)×10⁵ CFU/mL at light energy density of 175 and 200 J/cm² respectively, which were significantly lower than those of control group, single drug group, single light group and other low energy density ALA-PDT group (P<0.05). Conclusion ALA-PDT exerted fungicidal effect against Trichophyton tonsurans granuloma isolate, which might provide a theoretical basis for the clinical treatment of dermatophytes.

Key words: photodynamic therapy, Trichophyton tonsurans, Majocchi granuloma, dermatophytes

中图分类号:

R379.2

吴倩倩, 石磊, 张海艳, 张玲琳, 高志琴, 杨虹, 杨连娟, 王秀丽. 5-氨基酮戊酸光动力疗法体外抑制断发毛癣菌肉芽肿株增殖研究[J]. 中国真菌学杂志, 2018, 13(3): 139-143.

WU Qian-qian, SHI Lei, ZHANG Hai-yan, ZHANG Ling-lin, GAO Zhi-qin, YANG Hong, YANG Lian-juan, WANG Xiu-li. In vitro study of the 5-aminolevulinic acid photodynamic therapy for Trichophyton tonsurans strain isolated from granuloma[J]. Chinese Journal of Mycology, 2018, 13(3): 139-143.

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