超声联合两性霉素B纳米粒对白念珠菌生物膜杀菌效果评价

中国真菌学杂志　 2018, Vol. 13 　Issue (3): 134-138.

超声联合两性霉素B纳米粒对白念珠菌生物膜杀菌效果评价

杨敏¹, 谢霜¹, 董宇¹, 杜永洪¹, 李岱容²

1. 重庆医科大学生物医学工程学院省部共建国家重点实验室培育基地-重庆市超声医学工程重点实验室重庆市生物医学工程学重点实验室, 重庆 400016;
2. 重庆医科大学附属第一医院呼吸与危重症科, 重庆 400016

收稿日期:2018-01-03 出版日期:2018-06-28 发布日期:2018-06-28
通讯作者: 李岱容,E-mail:lidairong@126.com E-mail:lidairong@126.com
作者简介:杨敏,女(汉族),硕士研究生在读.E-mail:943640119@qq.com
基金资助:
重庆市基础科学与前沿技术研究专项一般项目（csct2016jcyjA0098）；重庆市社会事业与民生保障专项一般项目（cstc2016shmszx130029）

Evaluation of fungicidal effect of low frequency ultrasound and amphotericin B-loaded nanoparticles on C. albicans biofilm

YANG Min¹, XIE Shuang¹, DONG Yu¹, DU Yong-hong¹, LI Dai-rong²

1. State Key Laboratory of Ultrasound Engineering in Medicine Co-Founded by Chongqing and the Ministry of Science and Technology, College of Biomedical Engineering, Chongqing Key Laboratory of Biomedical Engineering, Chongqing Medical University, Chongqing Collaborative Innovation Center for Minimally-invasive and Noninvasive Medicine, Chongqing 400016, China;
2. First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China

Received:2018-01-03 Online:2018-06-28 Published:2018-06-28

摘要/Abstract

摘要：

目的评价低频超声联合两性霉素B纳米粒对白念珠菌生物膜的体外协同杀菌效果评价。方法采用双乳化法制备两性霉素B纳米粒（amphotericin B-loaded nanoparticles，AmB-NPs）。XTT减低法评价经超声与AmB-NPs联合作用后对成熟生物膜活性的影响，激光共聚焦显微镜观察生物膜形态学改变，并检测生物膜分泌蛋白酶和磷脂酶活力。结果与对照组及游离AmB药物相比，超声联合AmB-NPs能明显降低生物膜活性（P<0.01）；生物膜厚度变薄，结构疏松，蛋白酶和磷脂酶活力明显下降。结论低频超声联合AmB-NPs对白念珠菌生物膜有显著的协同抗菌作用。

关键词: 低频超声, 白念珠菌生物膜, 两性霉素B, 纳米粒, 协同抗菌

Abstract:

Objective To evaluate the synergistic fungicidal effect of low frequency ultrasound combined with amphotericin B-loaded nanoparticles (AmB-NPs) on C. albicans biofilm in vitro. Method AmB-NPs were prepared by a polylactic-co-glycolic acid (PLGA) double emulsion method. XTT reduction method was performed to evaluate the effect of ultrasound with AmB-NPs on the activity of mature biofilm. The morphology of biofilm was observed by laser scanning confocal microscope. The protease and phospholipase activities of biofilm were detected by UV spectrophotometer. Results The particle size and shape of AmB-NPs were uniform. The biofilm activity of C. albicans decreased significantly after the joint action of ultrasonic irradiation and AmB-NPs compared with the control group (P<0.01). Simultaneously, the biofilm became thin and loose, with significant decreased activities of protease and phospholipase aenzyme. Conclusion Low-frequency ultrasound combined with AmB-NPs showed significant synergistic antifungal effect against C. albicans biofilm in vitro.

Key words: low frequency ultrasound, C.albicans biofilm, amphotericin B, nanoparticles, synergistic fungicidal

中图分类号:

杨敏, 谢霜, 董宇, 杜永洪, 李岱容. 超声联合两性霉素B纳米粒对白念珠菌生物膜杀菌效果评价[J]. 中国真菌学杂志, 2018, 13(3): 134-138.

YANG Min, XIE Shuang, DONG Yu, DU Yong-hong, LI Dai-rong. Evaluation of fungicidal effect of low frequency ultrasound and amphotericin B-loaded nanoparticles on C. albicans biofilm[J]. Chinese Journal of Mycology, 2018, 13(3): 134-138.

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