实时荧光定量PCR检测马尔尼菲篮状菌病组织样本中菌载量的研究

中国真菌学杂志　 2018, Vol. 13 　Issue (2): 71-74.

实时荧光定量PCR检测马尔尼菲篮状菌病组织样本中菌载量的研究

郑艳青^1,2, 史娜娜^1,3, 曹存巍^1,3

1. 广西医科大学第一附属医院皮肤性病科, 南宁 530021;
2. 南宁市第四人民医院, 南宁 530023;
3. 广西艾滋病防治研究重点实验室, 南宁 530021

收稿日期:2017-11-29 出版日期:2018-04-28 发布日期:2018-04-28
通讯作者: 曹存巍,E-mail:caocunwei@yeah.net E-mail:caocunwei@yeah.net
作者简介:郑艳青,女(汉族),硕士研究生在读.E-mail:dryqzheng@yeah.net
基金资助:
国家自然科学基金（81571971，81271804）

The research of quantitative real-time PCR testing different specimen in diagnosis of talaromycosis

ZHENG Yan-qing^1,2, SHI Na-na^1,3, CAO Cun-wei^1,3

1. Department of Dermatology and Venereology, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021;
2. The Fourth People's Hospital of Nanning, Nanning 530023;
3. Guangxi Key Laboratory of AIDS Prevention and Treatment, Nanning 530021

Received:2017-11-29 Online:2018-04-28 Published:2018-04-28

摘要/Abstract

摘要：

目的实时荧光定量PCR测定马尔尼菲篮状菌病组织样本中马尔尼菲篮状菌的载量。方法收集马尔尼菲篮状菌病患者的皮肤病理切片、淋巴结活检组织，提取总DNA，采用实时荧光定量PCR技术检测样本中马尔尼菲篮状菌载量。结果皮肤病理切片、淋巴结活检组织中马尔尼菲篮状菌载量阳性。病理切片平均菌载量3.01×10⁴ copies（1.07×10⁴~7.26×10⁴ copies），淋巴结活检组织中马尔尼菲篮状菌载量4.68×10⁵ copies。结论荧光定量PCR能高效检测皮肤病理切片、淋巴结活检组织中马尔尼菲篮状菌载量，对马尔尼菲篮状菌病诊断有一定的价值。

关键词: 马尔尼菲篮状菌病, 实时荧光定量PCR, 分子诊断, 病理切片, 淋巴结活检组织

Abstract:

Objective To evaluate real-time fluorescence quantitative PCR (qPCR) in determining the load of Talaromyces marneffei in different samples of talaromycosis marneffei (TSM). Methods The loads of Talaromyces marneffei in pathological slices, biopsy tissues from suspected patients of TSM were measured by means of qPCR. Results The average load of Talaromyces marneffei in pathological slices was 3.01×10⁴ copies (1.07×10⁴~7.26×10⁴ copies), the load of Talaromyces marneffei in biopsy tissues was 4.68×10⁵ copies. Conclusion This study showed that qPCR determining the load of Talaromyces marneffei in pathological slices and biopsy tissues could be a useful method in TSM diagnosis.

Key words: talaromycosis marneffei (TSM), qPCR, diagnosis, pathological slices, biopsy tissues

中图分类号:

R519.8

郑艳青, 史娜娜, 曹存巍. 实时荧光定量PCR检测马尔尼菲篮状菌病组织样本中菌载量的研究[J]. 中国真菌学杂志, 2018, 13(2): 71-74.

ZHENG Yan-qing, SHI Na-na, CAO Cun-wei. The research of quantitative real-time PCR testing different specimen in diagnosis of talaromycosis[J]. Chinese Journal of Mycology, 2018, 13(2): 71-74.

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