白头翁汤正丁醇提取物对小鼠外阴阴道念珠菌病的治疗作用研究

中国真菌学杂志　 2017, Vol. 12 　Issue (6): 337-341.

白头翁汤正丁醇提取物对小鼠外阴阴道念珠菌病的治疗作用研究

段强军¹, 云云¹, 陈志珍¹, 邵菁¹, 汪天明², 汪长中¹

1. 安徽中医药大学中西医结合临床学院, 合肥 230038;
2. 安徽省中医药科学院中西医结合研究所, 合肥 230038

收稿日期:2017-07-07 出版日期:2017-12-28 发布日期:2017-12-28
通讯作者: 汪长中,E-mail:ahwcz63@sina.com E-mail:ahwcz63@sina.com
作者简介:段强军,男(汉族),硕士,讲师.E-mail:970813700@qq.com
基金资助:
国家自然科学基金（81573725，81774034）；安徽省教育厅科研重点项目（KJ2015A030，KJ2017A301）

Butyl alcohol extract of BaiTouWeng decoction inhibit vulvovaginal candidiasis in murine model via down-regulating NLRP3 inflammasome

DUAN Qiang-jun¹, YUN yun¹, CHEN Zhi-zhen¹, SHAO Jing¹, WANG Ting-ming², WANG Chang-zhong¹

1. School of Integrated Traditional and Western Medicine, Anhui University of Chinese Medicine, Hefei 230038, China;
2. Integrated Traditional and Western Medicine, Anhui Academy of Chinese Medicine, Hefei 23008, China

Received:2017-07-07 Online:2017-12-28 Published:2017-12-28

摘要/Abstract

摘要：

目的探讨白头翁汤正丁醇提取物（Butyl alcohol extract of BaiTouWeng，BAEB）对小鼠外阴阴道念珠菌病（vulvovaginal candidiasis，VVC）的治疗作用。方法雌性昆明种小鼠72只，随机分为正常组、模型组、白头翁汤正丁醇提取物高剂量、中剂量、低剂量与氟康唑组，皮下注射雌二醇致小鼠假发情期，再阴道接种白念珠菌2×10⁶ CFU/mL，药物治疗7 d。采用涂布法检测VVC小鼠的阴道真菌载荷量；HE染色法观察阴道组织的病理学改变；ELISA法检测小鼠阴道组织的IL-1β、IL-18的含量；qRT-PCR法检测小鼠阴道组织中NLRP3、ASC、caspase-1的mRNA表达。结果与正常组相比，模型组小鼠阴道黏膜层完全破坏，大量炎症细胞浸润；与模型组比较，BAEB组黏膜呈现不同程度的改善。与正常组相比较，模型组小鼠阴道组织IL-1β、IL-18含量升高，真菌载量明显升高，均具有统计学意义（P<0.001）。阴道组织中TLR2、TLR4、NLRP3、ASC、caspase-1的mRNA含量分别上调，差异均有统计学意义（P<0.001）。与模型组相比较，BAEB治疗组IL-1β、IL-18含量均降低，差异均有统计学意义（P<0.001）。结论白头翁汤正丁醇提取物对小鼠外阴阴道念珠菌病具有治疗作用。

关键词: 外阴阴道念珠菌病, NLRP3炎症小体, 白头翁汤正丁醇提取物, 白念珠菌

Abstract:

Objective To study the effects of Butyl alcohol extract of BaiTouWeng decoction (BAEB) on vulvovaginal candidiasis (VVC) in murine model.Methods Seventy-two female mice were randomly divided into normal group,model group,BAEB high-dose group,BAEB middle-dose group,BAEB low-dose group,and fluconazole group.The mice were firstly injected with estradiol to induce oestrum,and then intravaginally administrated with 2×10⁶ CFU/mL Candida albicans.After 7-day treatment,The fungal load (CFU/mL) was assessed from vaginal lavage fluid,the pathological changes of vaginal tissues were observed by HE staining.The levels of IL-1β and IL-18 in vaginal tissues were detected by ELISA.The mRNA expressions of NLRP3,ASC,caspase-1 were analyzed by qRT-PCR.Results Compared with the normal group,the vaginal mucosa of the model group was completely destroyed,and the infiltrations of plenty of inflammatory cells were observed.Compared with the model group,BAEB groups showed improvements to varying degrees.The levels of IL-1β and IL-18 in the model group were significantly higher than those in the normal group (P<0.001).The mRNA expressions of TLR2,TLR4,NLRP3,ASC and caspase-1 were up-regulated significantly (P<0.001).The levels of IL-1β and IL-18 in the BAEB groups were much lower than those in the control group (P<0.001).Conclusion The BAEB could be used to treat the vulvovaginal candidiasis in mice.

Key words: vulvovaginal candidiasis (VVC), NLRP3 inflammasome, Butyl alcohol extract of BaiTouWeng (BAEB), Candida albicans

中图分类号:

R519.3

段强军, 云云, 陈志珍, 邵菁, 汪天明, 汪长中. 白头翁汤正丁醇提取物对小鼠外阴阴道念珠菌病的治疗作用研究[J]. 中国真菌学杂志, 2017, 12(6): 337-341.

DUAN Qiang-jun, YUN yun, CHEN Zhi-zhen, SHAO Jing, WANG Ting-ming, WANG Chang-zhong. Butyl alcohol extract of BaiTouWeng decoction inhibit vulvovaginal candidiasis in murine model via down-regulating NLRP3 inflammasome[J]. Chinese Journal of Mycology, 2017, 12(6): 337-341.

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