两株白色不产孢烟曲霉形态学及毒力研究

中国真菌学杂志　 2017, Vol. 12 　Issue (2): 69-73.

两株白色不产孢烟曲霉形态学及毒力研究

姜媛¹, 张彩云¹, 王逢源¹, 孔庆涛¹, 张征¹, 曾秋琼¹, 龙南彪², 桑红¹

1. 南京军区南京总医院皮肤科, 南京 210002;
2. 南京师范大学生命科学学院, 南京 210046

收稿日期:2017-02-06 出版日期:2017-04-28 发布日期:2017-04-28
通讯作者: 桑红,E-mail:sanghong@nju.edu.cn E-mail:sanghong@nju.edu.cn
作者简介:姜媛,女(汉族),硕士研究生在读.E-mail:njujyuan@126.com
基金资助:
国家自然科学基金（81330035，81371782）

Phenotypic characterization and virulence of two white nonsporulating Aspergillus fumigatus isolates

JIANG Yuan¹, ZHANG Cai-yun¹, WANG Feng-yuan¹, KONG Qing-tao¹, ZHANG Zheng¹, ZENG Qiu-qiong¹, LONG Nan-biao², SANG Hong¹

1. Department of Dermatology, Jinling Hospital, Medical School of Nanjing University, Nanjing General Hospital of Nanjing Military Region, PLA, Nanjing 210002, China;
2. Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Microbiology, College of Life Sciences, Nanjing Normal University, Nanjing 210046, China

Received:2017-02-06 Online:2017-04-28 Published:2017-04-28

摘要/Abstract

摘要：

目的将先后发现的两株白色不产孢的烟曲霉A1j与A2j进行对比，观察其在形态学和毒力方面是否有差异。方法通过真菌基因组DNA的保守序列ITS和β-tub的测序对A2j进行菌种鉴定；采用小培养方法在显微镜下比较A1j、A2j及标准株Af293的菌丝形态；观察A1j和A2j在不同培养时间和不同培养基上生长的菌落形态；在小鼠模型上测试其毒力变化。结果通过ITS和β-tub的测序A2j被鉴定为烟曲霉；小培养发现A1j和A2j与标准菌株Af293不同，两者均为不产孢的烟曲霉，且两者生长速度明显不同；培养不同的时间和不同的培养基均不能诱导A1j和A2j产色和产孢，但是50℃下，A1j被限制生长，而A2j却能生长；在免疫抑制的小鼠模型中，与标准菌株Af293相比，A1j的毒力下降，但A2j小鼠的死亡数量、死亡速度与Af293相当，表明A2j的毒力并没有降低。结论新发现的白色不产孢烟曲霉A2j与A1j在生长速度、耐热性和毒力方面均不同。

关键词: 烟曲霉, 鉴定, 孢子形成, 侵袭性曲霉病模型

Abstract:

Objective In this study, another white nonsporulating A.fumigatus strain (named as A2j) was compared with reported nonsporulating strain A1j in terms of morphology and virulence.Methods A2j was confirmed by DNA sequencing of internal transcribed spacer (ITS) regions and partialb-tubulin genes.We incubated A1j and A2j through the slide culture method to compare them under a microscope.We observe the morphology of A1j and A2j at different times or on various medium types.An immunosuppressed mice model was created to compare the virulence of A1j, A2j and Af293.Results A2j was accurately identified as A.fumigatus by ITS and β-tubulin sequencing.Both A1j and A2j showed abnormal germination under the microscope compared to those of the reference strain Af293 by the slide culture method.Neither increased time nor different medium types could stimulate the formation of spores and pigment.A1j cannot grow and A2j can grow at 50℃.A1j is hypovirulentin immunosuppressed mice model;however, A2j is virulent compared to the standard strain Af293.Conclusion The newly A.fumigatus A2j and A1j have significant differences in growth rate, thermotolerance and virulence.

Key words: Aspergillus fumigatus, Identification, Sporulation, Invasive aspergillosis model

中图分类号:

R379.6

姜媛, 张彩云, 王逢源, 孔庆涛, 张征, 曾秋琼, 龙南彪, 桑红. 两株白色不产孢烟曲霉形态学及毒力研究[J]. 中国真菌学杂志, 2017, 12(2): 69-73.

JIANG Yuan, ZHANG Cai-yun, WANG Feng-yuan, KONG Qing-tao, ZHANG Zheng, ZENG Qiu-qiong, LONG Nan-biao, SANG Hong. Phenotypic characterization and virulence of two white nonsporulating Aspergillus fumigatus isolates[J]. Chinese Journal of Mycology, 2017, 12(2): 69-73.

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